▎ 摘　　要

NOVELTY - Method for rapid and highly sensitive detection of sulfamethazine (SM2) in foods derived from animal, involves synthesizing nanocomplex material silver-graphene oxide (Ag-GO), producing silver mirror reaction through silver nitrate and aqueous ammonia, combining negatively charged oxygen-containing groups under the reducing action of glucose, reducing silver nanoparticles in the complex, comparing and analyzing the characterization results, adding silver-GO-Nf nanocomplex dispersion liquid on the electrode surface, drying, electro-depositing in a chloroauric acid solution, depositing gold nanoparticles modified on the surface of the nanocomplex, obtaining modified SPA, anti-SM2 and SM2 antigens to obtain self-assembled layer on the electrode surface, drawing the regression curve, adding actual pork liver samples, calculating recovery rate and relative standard deviation (RSD), detecting sample, preparing pork/pig liver sample solution, and placing immunosensor in the sample solution. USE - The method is useful for rapid and highly sensitive detection of SM2 in foods derived from animal (claimed). ADVANTAGE - The method provides large specific surface area and biocompatibility of GO to construction of sensors, enhances electrical signal, and improves sensitivity of the sensor. DETAILED DESCRIPTION - Method for rapid and highly sensitive detection of sulfamethazine (SM2) in foods derived from animal, involves synthesizing nanocomplex material silver-graphene oxide (Ag-GO), producing silver mirror reaction through silver nitrate and aqueous ammonia, electrostatically combining negatively charged oxygen-containing groups under the reducing action of glucose, reducing silver nanoparticles in the complex through a series of characterizations of GO and silver-GO by UV-Vis absorption spectroscopy (Uv-Vis), X-ray diffraction spectroscopy (XRD) and scanning electron microscopy (SEM), comparing and analyzing the characterization results, firstly adding silver-GO-Nf nanocomplex dispersion liquid on the electrode surface, drying at room temperature, electro-depositing in a chloroauric acid solution, depositing gold nanoparticles modified on the surface of the nanocomplex, obtaining modified SPA, anti-SM2 and SM2 antigens to obtain self-assembled layer on the electrode surface through condition optimization, detecting SM2 standard solution of different concentration, drawing the regression curve to calculate the standard equation, adding actual pork liver samples, calculating recovery rate and relative standard deviation (RSD), detecting sample, processing and preparing pork/pig liver sample solutions containing 1 ng/ml, 10 ng/ml and 20 ng/ml SM2, and placing immunosensor in the sample solution for detection.