▎ 摘　　要

NOVELTY - Homogeneous immunoassay method based on ortho-position strike effect and graphene oxide quenching acridinium ester chemiluminescence comprises using detection solution comprising DNA1-conjugate, DNA2-conjugate, acridine ester (AE)-labeled DNA3, and graphene oxide (GO) combined antioxidant (AOD). USE - The method is useful for homogeneous immunoassay based on ortho-position strike effect and graphene oxide quenching acridinium ester chemiluminescence. ADVANTAGE - The method: does not require separation and cleaning steps; has simple operation; greatly shortens the clinical test specimen turnover time (TAT); does not require blood samples to be centrifuged, loads whole blood and issues test report within 5 minutes; and realizes anti-interference chemiluminescence detection and immunodetection of small chemical molecules. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for device for homogeneous immunoassay method based on ortho-position strike effect and graphene oxide quenching acridinium ester chemiluminescence, comprising rotor measurement module, light source module, exciter compartment module, exciter pump, exciter heating module, display module and printing module respectively connected to the circuit board module and host computer software control module. The rotor measurement module, and rotor are provided multiple of detection hole and a detecting hole with incubation functions, after the incubation time is over, the detection hole is turned to the detection position, the window of the light source module is aligned with the detection hole, and the detection is completed. The light source module comprises photon counting detector and counting unit. The photon counting detector is a photon counting detector with a large sensitive area comprising end-window photomultiplier tube, a high-voltage power supply module and a comparative forming circuit. The counting unit comprises counting control circuit and host computer acquisition software. The counting unit is directly used in combination with a photon counting detector or a scintillation detector as a counter. The exciter compartment module is used to carry the exciter bottle. The exciter pump is a micro-injection pump, and the exciter reagent is injected into the detection hole reaction cup through the pipeline and the exciter pump. The exciter heating module is used to control the temperature of the exciter reagent in the pipeline to ensure the accuracy of the test results. The barcode scanning module comprises a radio frequency card barcode scanning device, through which reagent information and standard curves are introduced into the host computer. The display module is used to display and edit multiple parameters on the device. The printing module is used to output the inspection report. The circuit board module is used to control the operation of other modules.