▎ 摘　　要

NOVELTY - Conjugated protein comprises silk protein and other structural protein macromolecules through the interaction between the two types of protein molecules to form a protein complex, is new. The protein complex is then solidified and cross-linked by the β-fold of silk protein to form the complex protein particles. The mass ratio of the silk protein to the structural protein is 0.01-100 and the size of the composite protein particles is 10 nm-500µm. The other structural proteins include collagen, elastin, gelatin, albumin, keratin, insect cuticle protein, proteoglycan, protein polypeptide and/or aforementioned protein derivatives. USE - The conjugated protein is useful in preparing superficial skin and subcutaneous fillers, in plastic and cosmetic surgery, in preparing filling materials and medicines for bone/cartilage repair, repairing and filling of wounds in bone tissue, cartilage tissue, muscle, blood vessels (claimed). ADVANTAGE - The hydrogel has mechanical characteristics of high mechanical strength and deformability, and long degradation period, and broad application prospects in the medical field. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for 1. composite protein particle assembling hydrogel material i.e. self-assembled from the composite protein particles to form a continuous porous colloidal gel network through the electrostatic interaction, hydrogen bond interaction and hydrophobic interaction on the surface of the particles; when the composite particle size is 10 nm-5 µm, where the volume fraction of the composite protein particles in the total volume of the hydrogel is 2-120 v/v% and the volume fraction of the composite particles in the total volume of the hydrogel is 40-120 v/v% when the particle size is more than 5 μm; 2. core-shell structure composite protein particle, comprising nanoparticles, where the shell layer is composed of silk protein and other structural protein macromolecules through the β folding of silk protein to realize cross-linking and compounding in the particle to form a shell layer, the mass ratio of the silk protein to the structural protein is 0.01-100, the mass ratio of the core nanoparticle to the shell composite protein material is 0.1-50, and the size of the composite particle is 10 nm-500 µm, the structural protein includes collagen, elastin, gelatin, albumin, keratin, silk protein, insect cuticle protein, proteoglycan, protein polypeptide and/or aforementioned protein derivatives, the core layer nanoparticles are silica nanoparticles, magnesium lithium silicate nanoparticles, nanoclay particles, hydroxyapatite nanoparticles, iron oxide magnetic nanoparticles, barium titanate nanoparticles, graphene nanosheets, carbon nanotubes, bioglass nanoparticles, black phosphorus nanosheets, silk fibroin nanoparticles, polylactic acid nanoparticles, polyethylene nanoparticles, and/or polystyrene nanoparticles; 3. Core-shell structure composite protein particle assembled hydrogel material i.e. self-assembled from the composite protein particles through electrostatic interaction, hydrogen bond interaction and hydrophobic interaction on the particle surface to form a continuous porous colloidal gel network, where the volume fraction of colloidal particles accounting for the total volume of the hydrogel is 2- 120 v/v.% when the particle size is 10 nm-5 µm and the volume fraction of the colloid particles in the granular hydrogel material to the total volume of the hydrogel is 50-120 v/v.% when the particle size is more than 5 µm; 4. preparing the conjugated protein; and 5. use of conjugated protein in preparing superficial skin and subcutaneous fillers, preferably in plastic and cosmetic surgery, and in preparing filling materials and medicines for bone/cartilage repair, where the above-mentioned gel is directly injected into the superficial skin or subcutaneous area when applied, the colloidal particles are blended with an aqueous solution to obtain a colloidal gel, fibroin and other structural protein composite particles reversibly self-assemble under the action of non-covalent bonds to form a continuous porous particle network, the colloidal particles are blended with an aqueous solution to obtain a colloidal gel, and then mixed with a cell suspension to obtain a cell-loaded colloidal gel in the preparation of bioprinting inks loaded with living cells, which extrudes the above ink into a 3D printing method to obtain a scaffold with a 3D structure, and obtain a cell-loaded printing scaffold, applied to the repair and filling of wounds or defects in bone tissue, cartilage tissue, muscle, blood vessels, the medicine ingredients are vitamins, amino acids, mineral elements, microecological regulators, growth factors, low molecular weight medicines, protein macromolecular medicines, nucleic acid medicines (e.g. mRNA), antibiotics, hormonal medicines, anesthetic medicines, antiviral medicines, antibacterial medicines, anticancer medicines, immunomodulatory medicines and/or living cells, the colloidal gel is injected directly into the bone/cartilage defect area when applied, fibroin and other structural protein composite particles reversibly self-assemble to form a continuous porous particle network, which stays stably at the bone defect site and acts as a scaffold material for bone repair and regeneration and the rapid hemostasis sealing powder comprises the composite protein particle powder when applied.