▎ 摘　　要

NOVELTY - Method for preparation heterostructure complex double-layer porous dressing involves mixing silk fibroin aqueous solution with conductive nanoparticle dispersion, adding antibacterial agent, then mixing, pouring into a mold, drying to obtain silk fibroin/reduced conductive nanoparticle complex membrane, post-processing the complex membrane with an aqueous ethanol solution, drying, immersing the dried complex membrane in deionized water, performing ultrasonic treatment, drying, formulating sodium hydroxide solution, adding curcumin/Aloe gel, then dripping into magnesium nitrate hexahydrate and aluminum nitrate nonahydrate mixed solution, dripping mixed solution of sodium carbonate/sodium hydroxide, centrifuging, washing, drying, dispersing in water, mixing with an aqueous solution of silk fibroin, concentrating, using the mixed solution and complex membrane as a receiving electrode for electrospinning and post-processing the heterostructure complex double-layer porous dressing. USE - The method is useful for preparation heterostructure complex double-layer porous dressing for promoting wound healing. ADVANTAGE - The composite double-layer porous dressing has good sterilizing and anti-inflammatory effect, and provides good promoting effect to the skin. The outer layer material of the dressing can well control the release of the anti-bacterial agent by electrical stimulation. The inner layer hybrid fiber film has a function release antiinflammatory medicine. The silk fibroin is a rich natural of protein, has good biological compatibility and biological degradability, is ideal selection as dressing base material, and is good for improving the conductivity and mechanical strength of the silk fiber film. DETAILED DESCRIPTION - Method for preparation heterostructure complex double-layer porous dressing involves (a) mixing silk fibroin aqueous solution with conductive nanoparticle dispersion and sterilizing the mixed solution in an autoclave, (b) adding suitable amount of antibacterial agent to the mixed solution obtained in step (a), then mixing, pouring into a mold, and drying to obtain silk fibroin/reduced conductive nanoparticle complex membrane, (c) post-processing the complex membrane with an aqueous ethanol solution and drying, (d) immersing the dried complex membrane obtained in step (c) in deionized water, performing ultrasonic treatment, and then drying, (e) formulating sodium hydroxide solution, adding curcumin/Aloe gel, then dripping sodium hydroxide/curcumin/Aloe gel mixed solution to magnesium nitrate hexahydrate and aluminum nitrate nonahydrate mixed solution, and evenly stirring the mixed solution under nitrogen atmosphere, (f) formulating mixed solution of sodium carbonate/sodium hydroxide, then dripping to the mixed solution obtained in step (e), while keeping the pH of the solution at a certain value, continuing stirring, centrifuging, then washing with deionized water for several times, and drying to obtain Curcumin/Aloe gel coated layered double hydroxide nano-hybrid particles, (g) dispersing the nano-hybrid particles in water to obtain a nano-hybrid particle dispersion liquid, mixing with an aqueous solution of silk fibroin and concentrating the mixed solution to a certain concentration of silk fibroin, (h) using the mixed solution obtained in step (g) and complex membrane obtained in step (d) as a receiving electrode for electrospinning to prepare a heterostructure complex double-layer porous dressing and (i) post-processing the heterostructure complex double-layer porous dressing with an aqueous ethanol solution.