▎ 摘　　要

NOVELTY - Method for constructing and using Faraday cage-type electrochemiluminescence immunosensor for detecting histone acetyltransferase activity involves (a) mixing acetyltransferase p300 with polypeptide and acetyl-conezyme A (CoA) in phosphate-buffered saline (PBS), incubating in a water bath, applying catalytic reaction droplets on the surface of the gold electrode and incubating in a refrigerator to obtain peptide/gold electrode, (b) mixing chloroauric acid, cetyltrimethylammonium bromide (CTAB) and water, adding ascorbic acid and sodium hydroxide to obtain CTAB-coated gold nanoparticles, centrifuging, purifying, dispersing in an equal amount of water, adding graphene oxide ultrasonic dispersion to the solution, adding acetyl antibody (Ab), incubating and methylene blue (MB) and evenly stirring the mixture to obtain MB-gold nanoparticle/GO/acetyl Ab and (c) coating the MB-gold nanoparticle/graphene oxide/acetyl Ab onto the peptide/gold surface, incubating and immersing the sensor in PBS. USE - The method is useful for constructing Faraday cage-type electrochemiluminescence immunosensor for detecting histone acetyltransferase activity. DETAILED DESCRIPTION - Method for constructing and using Faraday cage-type electrochemiluminescence immunosensor for detecting histone acetyltransferase activity involves (a) mixing acetyltransferase p300 with polypeptide and acetyl-conezyme A (CoA) in 0.1 M phosphate-buffered saline (PBS) having pH of 7, incubating in a constant temperature water bath, applying catalytic reaction droplets on the surface of the gold electrode and incubating in a refrigerator at 4 degrees C to obtain peptide/gold electrode, (b) mixing chloroauric acid, cetyltrimethylammonium bromide (CTAB) and water, adding ascorbic acid to the reaction mixture, adding sodium hydroxide to obtain CTAB-coated gold nanoparticles, centrifuging, purifying, dispersing in an equal amount of water, then adding graphene oxide ultrasonic dispersion to the solution, leaving, then adding acetyl antibody (Ab), incubating and methylene blue (MB) and evenly stirring the mixture to obtain MB-gold nanoparticle/GO/acetyl Ab and (c) coating the MB-gold nanoparticle/graphene oxide/acetyl Ab onto the peptide/gold surface, incubating at room temperature and then immersing the sensor in 0.1 M PBS having pH of 7 for electrochemical square wave voltammetry (SWV) test.