▎ 摘　　要

NOVELTY - This utility model claims an electrochemical immune sensor for detecting HE4 concentration, comprising base layer, electrode layer and insulation layer, also provided with groove on insulation layer, inner groove provided with alkene-nanometer gold film, and coating with streptavidin, by double antibody sandwich theory for HE4 one anti-biotin, the capture antigen of horseradish peroxidase mark and the HE4 second resistance in turn embellished on graphene nano gold film, be made of the the electrochemical immune sensor, this sensor combination of specificity and electrochemical immune reaction and detection sensitivity, using nano gold, graphene and so on novel nano materials, promoting electron transfer, which greatly increasing sensitivity of sensor detection. The prepared sensor is with simple operation, suitable for carrying, low detection cost.