▎ 摘　　要

NOVELTY - Method for preparing double-signal response-rate screen-printed electrode immunosensor involves adding solution of copper (III) sulfide-cuprous sulfide/graphene-loaded gold nanoparticles to the surface of the screen printing working electrode, continuously adding carcinoembryonic antigen (CEA)-capture antibody, washing electrode, adding e.g. toluidine blue/carboxyl gold nanoparticle functionalized mesoporous ceria/carboxymethyl chitosan/ionic liquid labeled antibody solution, incubating, washing and drying the electrode, and then storing in refrigerator. USE - The method is useful for preparing double-signal response-rate screen-printed electrode immunosensor (claimed). ADVANTAGE - The method enables to improve analytical performance, clinical reliability and accuracy. DETAILED DESCRIPTION - Method for preparing double-signal response-rate screen-printed electrode immunosensor involves (a) adding 4.5 mu l solution of copper (III) sulfide-cuprous sulfide/graphene-loaded gold nanoparticles (0.3-1.6 mg/ml) to the surface of the screen printing working electrode, and then drying at room temperature, (b) continuously adding 4.5 mu l carcinoembryonic antigen (CEA)-capture antibody (8-12 mu g/ml) in a dropwise manner to the modified electrode surface, drying the modified electrode surface at 4 degrees C, and then washing using ultrapurified water, (c) adding 4.5 mu l 0.05-0.15 %mass bovine serum albumin solution to block nonspecific active sites, drying the modified electrode surface at 4 degrees C, and then washing using ultrapurified water, (d) adding 4.5 mu l different concentrations of CEA solution (0.001-100 ng/ml) in a dropwise manner to the surface of the modified electrode, drying the modified electrode surface at 4 degrees C, and then washing using ultrapurified water, and (e) adding 4.5 mu l solution of toluidine blue/carboxyl gold nanoparticle functionalized mesoporous ceria/carboxymethyl chitosan/ionic liquid labeled antibody at a concentration of 0.8-2.4 mg/ml in a dropwise manner on the electrode, incubating at room temperature for 1 hour, washing and drying the electrode, and then storing in refrigerator.