▎ 摘　　要

NOVELTY - Preparing bacterial cellulose graphene composite modified glassy carbon electrode involves weighing 10-30 g of D-glucose, 2-10 g yeast powder, 2-10 g peptone, 1-2 g citric acid, 3-10 g magnesium sulfate and 1-5 g sodium chloride. A culture is prepared by adding Acetobacter xylophilus to culture for 2-5 days to obtain culture media. The obtained 500 ml culture media is mixed with 100 g sodium dicarboxylate, 0.2-1.0 g potassium phosphate and 1-5 wt.% ethanol at 25-30 degrees C to culture to obtain bacterial cellulose film. USE - Method for preparing bacterial cellulose graphene composite modified glassy carbon electrode for detecting nitrate (claimed). DETAILED DESCRIPTION - Preparing bacterial cellulose graphene composite modified glassy carbon electrode involves weighing 10-30 g of D-glucose, 2-10 g yeast powder, 2-10 g peptone, 1-2 g citric acid, 3-10 g magnesium sulfate and 1-5 g sodium chloride. A culture is prepared by adding Acetobacter xylophilus to culture for 2-5 days to obtain culture media. The obtained 500 ml culture media is mixed with 100 g sodium dicarboxylate, 0.2-1.0 g potassium phosphate and 1-5 wt.% ethanol at 25-30 degrees C to culture to obtain bacterial cellulose film. The obtained bacterial cellulose film is cut into small pieces to obtain small pieces of bacterial cellulose, which are removed in the ultra pure water to obtain medium residue. The obtained bacterial cellulose is boiled with 0.05-0.2 mol/Liter sodium hydroxide solution to obtain bacterial cellulose boiled mixture. The obtained bacterial cellulose is washed at neutral to obtain transparent bacterial cellulose. The obtained bacterial cellulose boiled mixture is subjected to freeze-drying to obtain dried mixture. The obtained dried mixture is mixed with graphene oxide in ultra-pure water or ethanol mixture to obtain bacterial cellulose graphene composite powder. The obtained powder is coated on the surface of the clean glassy carbon electrode and dried at room temperature to obtain the desired product. An INDEPENDENT CLAIM is included for a method for detecting nitrate by using bacterial cellulose graphene composite modified glassy carbon electrode, which involves: (A) preparing bacterial cellulose graphene composite modified glassy carbon electrode with the platinum wire and the saturated calomel electrode to obtain three-electrode system; (B) placing the obtained three-electrode system in nitric acid solution; (C) determing the catalytic performance of the bacterial cellulose graphene complex to nitrite by cyclic voltammetry; (D) placing the three electrode system placed in the phosphate buffer solution, dropping into different concentrations of sodium nitrite solution; (E) measuring the constant potential in different concentrations of sodium nitrite solution corresponding to the response current value, and the preparation of nitrite; and (F) obtaining the concentration of nitrite ions in the phosphate buffer solution to be measured by using the linear relationship diagram containing the phosphate buffer solution to obtain the desired product.