▎ 摘　　要

NOVELTY - Photoelectric detecting of oxygen vacancy titanium dioxide/graphene-based DNA methylation, comprises (i) preparing graphene oxide (GO) coating layer on the surface of ZnO by the layer-by-layer self-assembly method based on electrostatic interaction, reduction of GO to prepare oxygen-vacancy TiO2-x/G nanotube array electrodes; (ii) using EDAC (carbodiimide) as the coupling agent and benzotriazole (Bt) as the reaction intermediate, reacting the dihydroxybenzoic acid (PCA) reacts with the capture probe ssDNA with an amino modified at one end to form an amide bond and obtaining ssDNA-PCA molecules; (iii) immersing the TiO2-x/G nanotube array electrode in the solution of the prepared ssDNA-PCA molecules, assembling the electrode in the PCA solution again to form ssDNA-PCA /TiO2-x/G nanotube array electrode; (iv) denaturing the DNA methylation sample and (v) immersing the ssDNA-PCA/TiO2-x/G nanotube array electrode in the DNA methylation sample solution, and hybridizing. USE - The method is useful for photoelectric detecting of oxygen vacancy titanium dioxide/graphene-based DNA methylation. ADVANTAGE - The method is simple, fast and sensitive. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic diagram of a DNA-PCA/TiO2-x/G photoelectric response system for DNA methylation detection (Drawing includes non-English language text).