• 专利标题:   System used to diagnosis of Leptospira interrogans comprises screen printed paper electrode (SPPE) having amine linked capture probe immobilized on SPPE, amino linked single stranded DNA probe added to activated working surface of SPPE.
  • 专利号:   IN202211022145-A
  • 发明人:   VERMA V, KALA D, KAUSHAL A, KUMAR D
  • 专利权人:   UNIV SHOOLINI BIOTECHNOLOGY MANAGEMENT
  • 国际专利分类:   A61K039/02, B82Y015/00, C12Q001/6825, G01N027/30, G01N027/327
  • 专利详细信息:   IN202211022145-A 23 Dec 2022 G01N-027/327 202310 Pages: 23 English
  • 申请详细信息:   IN202211022145-A IN11022145 13 Apr 2022
  • 优先权号:   IN11022145

▎ 摘  要

NOVELTY - System comprises a screen printed paper electrode (SPPE) having an amine linked capture probe (Lipl-41) immobilized on the SPPE, where working surface the SPPE is washed several times using a phosphate-buffer saline to activate the working surface of the SPPE, and an amino linked single stranded DNA (ssDNA) probe added to the activated working surface of the SPPE and incubated in a humid chamber for 4 hours to form an ssDNA probe modified electrode, where the ssDNA probe modified electrode is washed using Tris-EDTA (TE) buffer to recapture excessive probe, a leptospiremic genomic DNA is denatured and hybridized with the ssDNA probe modified electrode for 10 minutes at a room temperature, and a cyclic voltammogram (CV) analysis is performed using methylene blue to compute sensibility of the system and impedimetric spectroscopy (EIS) studies is recorded using potassium ferricyanide to confirm hybridization event that occurs on the working surface of the SPPE. USE - The system is useful for: timely diagnosis of Leptospira interrogans (claimed). DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for timely diagnosis of Leptospira interrogans.