▎ 摘　　要

NOVELTY - A reduced graphene oxide/persimmon tannin/platinum-palladium composite nano material preparing method involves placing graphene in distilled water for ultrasonically dispersion to obtain a graphene suspension, adding ascorbic acid to the suspension, stirring, centrifuging and removing the supernatant in the suspension to obtain reduced graphene oxide. The persimmon tannin is passed into the reduced graphene oxide solution ultrasonically to make the reduced graphene oxide/ persimmon tannin suspension, chloroplatinic acid and palladium(II) nitrate is added to the suspension, ascorbic acid is added to the suspension, stirred, centrifuged and removed the supernatant from the suspension and the precipitate is washed with ultrapure water and centrifuged to obtain the reduced graphene oxide/persimmon tannin/platinum-palladium composite nano material. USE - Method for preparing reduced graphene oxide/persimmon tannin/platinum-palladium composite nano material for detecting anhydroglucitol. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a method for detecting 1,5-anhydroglucitol, which involves placing a screen printed electrode in a sulfuric acid solution for cyclic voltammetry scanning to obtain an activated screen printed electrode and rinsing with pure water, performing a constant potential deposition of gold on the screen printed electrode after activation and washing the electrode, pipetting the suspension of reduced graphene oxide/persimmon tannin/platinum-palladium composite nano material, adding the nano material to the surface of the pretreated electrode and incubating the electrode in the incubator to obtain a working electrode modified with reduced graphene oxide/persimmon tannin/platinum-palladium composite nanomaterial, adding 0.5mg/mL PROD solution to the surface of 1.0 mg/mL reduced graphene oxide/persimmon tannin/platinum-palladium composite nanomaterial modified electrode and incubating in air, which is the electrochemical biosensing interface of the constructed 1,5-anhydroglucitol, adding 1,5-anhydroglucitol standard solution to the 1,5-anhydroglucitol electrochemical biosensing interface constructed and incubating in the incubator for a period of time to obtain 1,5-anhydroglucitol electrochemical biosensor, immersing the working electrode into the phosphate-buffered saline solution, scanning the electrode with a differential pulse voltammetry using an electrochemical workstation and recording the response current value of the sensor, drawing a working curve and calculating the minimum detection limit of the method according to the relationship between the current response value of the sensor and the concentration of 1,5-anhydroglucitol, adding an actual sample to be tested at the 1,5-anhydroglucitol electrochemical biosensing interface, incubating the sample in the incubator for a period of time, washing the sample with phosphate-buffered saline solution to obtain a working electrode and drying the electrode, passing the working electrode into the phosphate-buffered saline solution and using the differential pulse voltammetry scan of the electrochemical workstation to record the peak current and testing the concentration of 1,5-anhydroglucitol in the actual sample according to the standard curve.