▎ 摘　　要

NOVELTY - Method of detecting presence of an analyte in an environment using biological field-effect transistor (BioFET) involves exposing a three-dimensional (3D) graphene layer biofunctionalized with a biological recognition element to an external environment that includes a target analyte, providing a well region containing an electrolyte solution configured to retain the target analyte, allowing the target analyte to disperse throughout the electrolyte solution contained in the well region and bind with the biological recognition element, detecting a change in an electric current, an electrical conductivity, or an electrical resistance of the 3D graphene layer in response to the target analyte binding with the biological recognition element, determining a presence of the target analyte based on the detected change in electric current, electrical conductivity, or electrical resistance of the 3D graphene layer, and outputting an indication of the determined presence of the target analyte. USE - The method is useful for detecting presence of an analyte in an environment using BioFET. The target analyte is one or more of a nucleic acid or a protein (claimed). ADVANTAGE - The method exhibits adverse ionic screening effects at physiologically relevant conditions, and accurately detects the presence and concentration levels of analytes. The 3D graphene provides an improved surface area to volume ratio without suffering impediments resulting from impurities residing on exposed carbonaceous surfaces of the pristine 3D graphene. DETAILED DESCRIPTION - Method of detecting presence of an analyte in an environment using biological field-effect transistor (BioFET) involves exposing a three-dimensional (3D) graphene layer biofunctionalized with a biological recognition element to an external environment that includes a target analyte, where the 3D graphene layer operates as a channel for the BioFET, providing a well region containing an electrolyte solution configured to retain the target analyte, allowing the target analyte to disperse throughout the electrolyte solution contained in the well region and bind with the biological recognition element, detecting a change in one or more of an electric current, an electrical conductivity, or an electrical resistance of the 3D graphene layer in response to the target analyte binding with the biological recognition element, determining a presence of the target analyte based on the detected change in electric current, electrical conductivity, or electrical resistance of the 3D graphene layer, and outputting an indication of the determined presence of the target analyte.