• 专利标题:   Sensor used to detect severe acute respiratory syndrome (SARS)-coronavirus (COV)-2 in sample comprises graphene layer, many binding agents coupled to graphene layer to generate functionalized graphene layer, and many electrical conductors.
  • 专利号:   US2021293816-A1, WO2021189041-A1, AU2021239399-A1, CA3170884-A1, KR2022156058-A, EP4121762-A1, CN115836226-A, JP2023517504-W
  • 发明人:   FOTOUHI M, PATEL N, GREENFIELD E A, ABEDI M, MOLLAAGHABABA R, NAWANA N, ME M A, MOLAGABABA R, POTUHI M
  • 专利权人:   GRAPHENEDX INC, GRAPHENEDX INC, GRAPHENEDX INC, GRAPHENEDX INC
  • 国际专利分类:   G01N027/12, G01N033/569, G01N033/543, C12Q001/6825, C12Q001/6834, C12Q001/6888, C12Q001/70, G01N027/02, G01N027/414, G01N033/483, G01N033/53
  • 专利详细信息:   US2021293816-A1 23 Sep 2021 G01N-033/569 202197 English
  • 申请详细信息:   US2021293816-A1 US208692 22 Mar 2021
  • 优先权号:   US992677P, US009209P, US023014P, US208692, CA3170884, CN80022882, KR736341

▎ 摘  要

NOVELTY - Sensor (400), comprises a graphene layer (14), many binding agents coupled to the graphene layer to generate a functionalized graphene layer, where the binding agents exhibit specific binding to at least one epitope of SARS-COV-2 and many electrical conductors electrically coupled to the functionalized graphene layer for measuring at least one electrical property of the functionalized graphene layer. The epitope is S or N protein of the SARS-COV-2. The binding agents are anti-SARS-CoV-2 antibodies. The sample comprises a biological sample, preferably blood sample. The binding agents are coupled to the graphene layer via many linkers. The linkers: are covalently attached at one end to the graphene layer and at another end to at least one epitope of SARS-COV-2; comprise 1-pyrenebutonic acid succinimidyl ester. The graphene layer is functionalized with many hydroxy. The binding agents are coupled: to hydroxy by many aldehyde moieties; or to the graphene layer via protein G. USE - The sensor is useful for detecting SARS-COV-2 in a sample (claimed). ADVANTAGE - The sensor: can be utilized at the point of sample collection by a lay person with minimal technical expertise; and utilizes antibodies, thus enhancing the detection of the SARS-COV-2 virus; and provides the detection results rapidly. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) detecting SARS-COV-2 in a biological sample; (2) fabricating a sensor for detecting antibodies reactive against SARS-COV-2 virus in a biological sample; and (3) a disposable cartridge for detecting SARS-COV-2 virus in a biological sample. DESCRIPTION OF DRAWING(S) - The figure shows the graphene-based sensor. Substrate (12) Silicon oxide layer (13) Graphene layer (14) Antibodies (16) Sensor (400)