▎ 摘　　要

NOVELTY - Preparing red fluorescein labeled graphene oxide involves dissolving fluorescein and rhodamine B in dimethyl sulfoxide to obtain solution A which is stored in dark. The mass ratio of fluorescein and rhodamine B in dimethylsulfoxide is (0.5-1)/(1-5) mole/ml. The thyroglobulin is added to aqueous sodium carbonate solution to reach the pH value of 8.5-9.5 and the solution is stirred at room temperature until complete dissolution to obtain solution B. The mass ratio of thyroglobulin and aqueous sodium carbonate is (0.1-0.5)/(10-20) mole/ml. USE - Method for preparing red fluorescein labeled graphene oxide (claimed). ADVANTAGE - The method enables to prepare red fluorescein labeled graphene oxide that is fast and produces notable influence. DETAILED DESCRIPTION - Preparing red fluorescein labeled graphene oxide involves dissolving fluorescein and rhodamine B in dimethyl sulfoxide to obtain solution A which is stored in dark. The mass ratio of fluorescein and rhodamine B in dimethylsulfoxide is (0.5-1)/(1-5) mole/ml. The thyroglobulin is added to aqueous sodium carbonate solution to reach the pH value of 8.5-9.5 and the solution is stirred at room temperature until complete dissolution to obtain solution B. The mass ratio of thyroglobulin and aqueous sodium carbonate is (0.1-0.5)/(10-20) mole/ml. The solution A is transferred to a syringe and the solution B is transferred to a container. The solution A in 1-5 volume parts is dropwise added to 10-20 volume parts of solution B under dark conditions to obtain solution C. The solution C is transferred to a dialysis bag, the dialysis bag is then immersed in an aqueous solution of sodium carbonate at the pH of 8.5-9.5 to carry out dialysis. The dialysis solution bag changes from turbid to clear and transparent solution which indicates the completion of dialysis. The dialysis bag is removed to obtain rhodamine B labeled thyroglobulin solution. The graphene oxide is dispersed in water to obtain a solution D of 1.5-2.5 mg/ml concentration. The rhodamine B labeled thyroglobulin solution is diluted in water upto the concentration of 3-6 mg/ml to obtain solution E. The solution D and solution E are mixed under low light conditions in the volume ratio of 1:(0.8-1.2) for 12-60 hours. The mixed solution is centrifuged to separate the solid to obtain red fluorescein labeled graphene oxide.