▎ 摘　　要

NOVELTY - Bionic nanoparticle based on fusion cancer cell membrane of mesenchymal stem cells, is claimed. The bionic nanoparticles are drug-loaded nanoparticles, which are covered by a layer of fusion cell membrane. The fusion membrane comprises a mesenchymal stem cell membrane and a cancer cell membrane, bionic nano drug delivery system that can target tumors and tumor bone metastases. USE - The bionic nanoparticle is useful for preparing tumor and bone metastatic tumor targeted therapeutic medicine (claimed). ADVANTAGE - The bionic nanoparticle: has strong targeting and good safety; can enhance the curative effect and reduces toxic and side effect; has high clinical practice; can accurately deliver the medicine to the tumor and tumor bone metastasis part by internal and external experiments of the body. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for preparing bionic nanoparticle based on fusion cancer cell membrane of mesenchymal stem cells comprising (1) preparing lipoic acid nano-particle lipoic acid and cysteine, dissolving in methanol, stirring and crosslinking at room temperature, nitrogen blowing, adding dichloromethane to dissolve, adding 1 % sodium cholate solution, ultrasonic emulsifying, adding double distilled water, stirring and centrifuging, using W ultra-filtration tube to centrifuge to obtain the purified lipoic acid nano-particle, (2) using solid phase synthesis method to synthesize LACL polypeptide, stirring LACL and cysteine in methanol and crosslinking in methanol, nitrogen blowing dry methanol, dissolving LACLss in double distilled water, self-assembly to form LACLs smicelle, (3) mixing LA-NP and LACLss, incubating and self-assembling to obtain LC nano particle, (4) preparing stearoyl poly peptide polymer SHRss by using the solid phase synthesis method to synthesize the stearoyl polypeptide, preparing SHR into solution by double distilled water, adding L-cysteine hydrochloride, adjusting pH to 7.0, stirring and dropping hydrogen peroxide, room temperature photophobic reaction and using dialysis bag in the distilled water to dialyze, collecting freeze-dried to obtain SHRss, (5) preparing PLGA nanoparticles by ultrasonic emulsification method, dissolving PLGA in dichloromethane, adding to PVA 124 solution, ultrasonic emulsifying, stirring at room temperature overnight, centrifuging, collecting PLGA nanoparticles precipitate, double-distilled water to obtain PLGA nanoparticles, (6) taking silicon dioxide and mono-dispersed mesoporous silicon dioxide nano-ball-star MSN, dissolving in double distilled water to obtain silicon dioxide nano-particle solution and MSN nano-particle solution, (7) dissolving the black phosphorus powder in double distilled water, ultrasonically processing to obtain the P-NP solution, (8) preparing graphene quantum dot polymer amino-terminated graphene quantum dot (GOD) solution and reducing sensitive amino crosslinking agent 3', 3'-dithio-bis(sulfonic acid succinimidyl propionate) (DTSSP), cross-linking and stirring overnight, and dialyzing in distilled water by dialysis bag, collecting freeze-dried to obtain GODss, (9) preparing peptide modified multi-wall carbon nano-tube (MHR): synthesizing H3R6 polypeptide by solid phase synthesis method, taking multi-wall carbon nano-tube (MWCNTs), adding 68 % nitric acid, stirring and refluxing, cooling to room temperature, diluting by distilled water and filtering and drying, weighing the purified MWCNTs into concentrated sulfuric acid/concentrated nitric acid mixed acid, reacting in water bath, centrifuging to remove the reaction liquid, freeze-drying to obtain the carboxylated MWCNT-COOH, weighing MWCNT-COOH to double distilled water containing 1-ethyl - (3-dimethylamino-propyl)carbonyl diimine hydrochloride (EDC) and N-NPshydroxyl succinimide (NHS), stirring at room temperature, feeding according to the mass ratio of MCWNT-COOH and H3R6 of 1: 30, stirring at room temperature, centrifuging to remove the reaction liquid, and freeze-drying to obtain product.