▎ 摘　　要

NOVELTY - Preparing electrochemical biosensor based on the butterfly effect involves adding the DNA tetrahedron 2, gold nanoparticles (AuNPs), methylene blue (MB), and 3D graphene composites to the electrode surface, and methylene blue (MB) is utilized as the signal output for electrochemical detection by square wave voltammetry when ATP is added to the surface of the electrode, subsequently, utilizing ALP to catalyze the consumption of ATP to adenosine, further this strategy is utilized to realize the analysis of ALP activity and its small molecule inhibitors, and based on this, a simple, stable, rapid, label-free, high-sensitivity, and high-selectivity electrochemical analysis method for ATP (ALP) is constructed. USE - Method for preparing electrochemical biosensor based on the butterfly effect for interacting with gold to form gold-sulfide bonds to fix the DNA tetrahedron 1 to the surface of the gold electrode. ADVANTAGE - The method enables to prepare electrochemical biosensor based on the butterfly effect, which realizes the analysis of ALP activity and its small molecule inhibitors. The electrochemical signal increases sharply, similar to the butterfly effect. The method is simple, stable, rapid, label-free, highly sensitive and highly selective electrochemical analysis method.