▎ 摘　　要

NOVELTY - Detection of heme involves weighing chlorhematin, dissolving in 0.01 mol/L sodium hydroxide solution, diluting with 0.01 M phosphate-buffered saline (PBS) solution and 0-20 mg/L protohemin solution, transferring to 1 L volumetric flask to obtain heme first mother liquor, where mass concentration of hemoglobin in mother solution is 0 mg/L, and marking as blank blood red mother solution; mixing graphene quantum dots with 0.01 M PBS solution, diluting to obtain 20 mg/L graphene quantum dot detection agent; and adding 4 mL heme mother liquor to 4.0 mL graphene quantum dots detection agent. USE - Method for detecting heme (claimed). ADVANTAGE - The method has simple operation, high detection sensitivity, low detection limit, and good stability. DETAILED DESCRIPTION - Detection of heme comprises: (A) weighing chlorhematin, dissolving in 0.01 mol/L sodium hydroxide solution, diluting with 0.01 M PBS solution and 0-20 mg/L protohemin solution, transferring to 1 L volumetric flask to obtain heme first mother liquor, where mass concentration of hemoglobin in mother solution is 0 mg/L, and marking as blank blood red mother solution; (B) mixing graphene quantum dots with 0.01 M PBS solution, diluting to obtain 20 mg/L graphene quantum dot detection agent; (C) adding 4 mL heme mother liquor to 4.0 mL graphene quantum dots detection agent to obtain 0-10 mg/mL heme mass concentration, and adding 4 mL absorbing blank heme mother liquid to 4 mL graphene quantum dot detection agent to get 0-10 mg/mL orceins blank sample; (D) shaking for 30 seconds, standing for 10 minutes, putting 2.0 mL sample into fluorescent cuvette, xenon lamp light source irradiating at 380-650 nm, taking blank sample solution, shaking for 30 seconds, standing for 10 minutes, putting 2.0 mL in cuvette, and xenon lamp light source irradiating at 380-650 nm; and (E) setting fluorescence intensity quenching value of sample liquid at 470 nm, determining correspondence relationship between quenching value and fluorescence intensity of the sample liquid at 470 nm, and measuring mass concentration of heme.