▎ 摘　　要

NOVELTY - Biomarker detection sensor (100) comprises a substrate, working electrode (104) formed by laser-scribing directly into substrate so that material of the substrate is transformed into graphene. A metal nanostructure (110) formed on a graphene surface of the working electrode, where the metal nanostructure is shaped as a tree with multiple branches extending away from the graphene surface. The sensor also comprises an aptamer for covering first surface area of metal nanostructure, a reference electrode (106), and a counter electrode (108). USE - Biomarker detection sensor used for rapid diagnosis at outpatient healthcare, hospitals, critical care centers, and home healthcare. ADVANTAGE - The biomarker detection sensor has low-cost, and shows accurate POC detection of disease biomarkers, which is critical for managing global health issues, and is easy-to-use. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) Method for making a biomarker detection sensor, involves providing a polyimide substrate; scribing with a laser beam into the polyimide substrate to form a graphene working electrode; depositing a metal by electrochemical deposition on the graphene working electrode to form a metal nanostructure that extends as a tree with branches from a surface of graphene working electrode; adding a biomarker and polymer to the surface of the graphene working electrode; and removing the biomarker to form corresponding cavities into the deposited polymer; and (2) System for determining a biomarker, comprises a biomarker detection sensor, a signal analyzer is configured to directly connect to the biomarker detection sensor to receive measurements and generate a signal indicative of the biomarker; and a portable computing device that receives the signal and displays the signal on a screen. DESCRIPTION OF DRAWING(S) - The drawing shows a laser-scribed graphene sensor that uses metal nanostructure with an aptamer for detecting biomarker Biomarker detection sensor (100) Working electrode (104) Reference electrode (106) Counter electrode (108) Metal nanostructure (110)