▎ 摘　　要

NOVELTY - Detecting chlorogenic acid using graphene quantum dots comprises (i) taking multiple equal parts of volume as V1, mixing graphene quantum dot solution of equal concentration C1 with different volumes of chlorogenic acid standard solution of known concentration, fixing volume of mixed solution to V2 with phosphate-buffered saline (PBS) buffer, carrying out reaction for 8-12 minutes, (ii) preparing blank test solution with volume of V2 and chlorogenic acid concentration, (iii) using fluorescence spectrophotometer at room temperature, scanning fluorescence intensity of each test solution and blank test solution under maximum emission wave, recording measured fluorescence intensity of blank solution, and fluorescence intensity of each test solution, calculating and recording difference, (iv) taking concentration of chlorogenic acid as abscissa, and drawing standard working curve, and (v) using fluorescence spectrophotometer for scanning and measuring fluorescence intensity. USE - The method is useful for detecting chlorogenic acid using graphene quantum dots. ADVANTAGE - The method: is environmental friendly; easy acquisition, simple operation, short time-consuming and sensitivity; and utilizes graphene quantum dots with high fluorescence yield. DETAILED DESCRIPTION - Detecting chlorogenic acid using graphene quantum dots comprises (i) taking multiple equal parts of volume as V1, mixing graphene quantum dot solution of equal concentration C1 with different volumes of chlorogenic acid standard solution of known concentration, fixing volume of mixed solution to V2 with phosphate-buffered saline (PBS) buffer, carrying out reaction for 8-12 minutes, and transferring to step (iii) as test solution, (ii) preparing blank test solution with volume of V2 and chlorogenic acid concentration of 0 according to method of step (i), where concentration of graphene quantum dots in blank test solution is same as concentration in each test solution in step (i), (iii) using fluorescence spectrophotometer at room temperature, scanning fluorescence intensity of each test solution obtained in step (i) and blank test solution obtained in step (ii) under maximum emission wave, recording measured fluorescence intensity of blank solution as F0, and fluorescence intensity of each test solution as F1, F2...Fn, calculating and recording difference between F0-Fn, which is quenching value of fluorescence intensity of graphene quantum dots by chlorogenic acid, (iv) taking concentration of chlorogenic acid in each test solution after constant volume in step (i) as abscissa, and drawing standard working curve with corresponding F0-F1, F0-F2...F0-Fn as ordinate, and (v) taking graphene quantum dot solution with same volume V1 and same concentration C1 as in step (i) and chlorogenic acid solution with unknown concentration and volume V3 to be testing, fixing the mixed solution to volume V2 with PBS buffer, carrying out reaction for 8-12 minutes, taking as test solution, using fluorescence spectrophotometer for scanning and measuring fluorescence intensity F of liquid to be testing, determining concentration C2 of chlorogenic acid in test solution according to standard working curve obtained in step (iv), and further converting concentration C3 of chlorogenic acid solution to be testing.