▎ 摘　　要

NOVELTY - Preparing sulfur-containing diphenylboronic acid (SDBA)-gold composite nanoenzyme involves preparing SDBA with a certain spatial distance. Gold composite nanoenzyme is loaded on the carrier by in-situ reduction method by dissolving chloroauric acid aqueous solution and the doping reagent in deionized water at room temperature to prepare a reaction solution, adding a reducing agent to the reaction solution for 0.5-2 hours, adding carrier and the reacting for 3-24 hours, washing final product followed by preparing SDBA-gold composite nanoenzyme by adding gold composite nanoenzyme, SDBA compound and absolute ethanol to deionized water, stirring at 20-50 degrees C for 10-20 hours, washing final product with centrifugal water to remove residual reactants to prepare SDBA-gold composite nanoenzyme, where mass-volume ratio of gold composite nanoenzyme, SDBA compound, absolute ethanol and deionized water is 1-2 mg:0.1-0.5 mg:1-2 ml:1-10 ml. USE - Method for preparing sulfur-containing diphenylboronic acid-gold composite nanoenzyme that is utilized for preparing a glucose potential sensor for detecting concentration of glucose in body fluids (all claimed). ADVANTAGE - The glucose potential sensor improves the selectivity of gold composite nano-enzyme to glucose, reduces the interference of other monosaccharide and biological macromolecule to the potential signal in the actual sample, has important significance in developing the intelligent toilet for family self-health monitoring. DETAILED DESCRIPTION - Preparing sulfur-containing diphenylboronic acid (SDBA)-gold composite nanoenzyme involves preparing SDBA with a certain spatial distance by suspending (2-formylphenyl)boronic acid and 4,4`-thiodiphenylamine in anhydrous methanol at room temperature to prepare a reaction solution, stirring reaction solution under nitrogen atmosphere for 2-5 hours, adding sodium borohydride aqueous solution dropwise to the nitrogen-filled reaction solution, and continuing reaction for 5-24 hours after quenching the reaction with saturated ammonium chloride aqueous solution, extracting organic phase with ethyl acetate, washing with water and brine, drying over sodium sulfate, and filtering reaction mixture and concentrating by a silica gel column to purify the reaction mixture to obtain the sulfur-containing diphenylboronic acid compound. Gold composite nanoenzyme is loaded on the carrier by in-situ reduction method by dissolving chloroauric acid aqueous solution and the doping reagent in deionized water at room temperature to prepare a reaction solution, adding a reducing agent to the reaction solution for 0.5-2 hours, adding carrier and the reacting for 3-24 hours, washing final product with centrifugal water to remove residual reactants to prepare gold composite nanoenzyme, where the mass ratio of chloroauric acid, doping reagent, reducing agent, carrier and deionized water is 1-5:0-50:1-20: 5-50: 200-1000, the carrier is carbon nanotubes, carbon spheres or graphene, the doping agent is chloroplatinic acid, cobalt chloride, iron chloride or nickel chloride and reducing agent is sodium citrate or sodium borohydride, and the types of doping reagents are 0, 1, 2, 3 or 4 types followed by preparing SDBA-gold composite nanoenzyme by adding gold composite nanoenzyme, SDBA compound and absolute ethanol to deionized water, stirring at 20-50 degrees C for 10-20 hours, washing final product with centrifugal water to remove residual reactants to prepare SDBA-gold composite nanoenzyme, where mass-volume ratio of gold composite nanoenzyme, SDBA compound, absolute ethanol and deionized water is 1-2 mg:0.1-0.5 mg:1-2 ml:1-10 ml. An INDEPENDENT CLAIM is also included for a method for preparing a glucose potential sensor based on SDBA-gold composite nanoenzyme, which involves spreading the SDBA-gold composite nanoenzyme on the surface of the basic electrode and drying at 4-37 degrees C for 0.5-5 hours, dripping the Nafion membrane solution with a concentration of 1-5% and drying it at 4-37 degrees C for 0.5-5 hours to obtain glucose potential sensor, and storing at 0-4 degrees C until use, where the thickness of SDBA-gold composite nanoenzyme is 30-100 nm, and the thickness of Nafion membrane is 0.5-5 mu m, the basic electrode is a glassy carbon electrode, a paper electrode, a gold electrode, a platinum electrode or a flexible electrode.