▎ 摘　　要

NOVELTY - Preparing chitosan oligosaccharide three dimensional graphene electrode comprises e.g. (i) adding chitosan oligosaccharide into graphene oxide aqueous solution, mixing uniformly, incubating, (ii) taking out the chitosan oligosaccharide-three-dimensional graphene hydrogel, freezing, placing the frozen chitooligosaccharides - three-dimensional graphene hydrogel in a freeze-dryer and freeze-drying, (iii) dicing the chitosan oligosaccharide-three-dimensional graphene airgel, taking copper wire, applying and exposing silver plastic with insulating silicone sealing insulation. USE - The graphene electrode is useful for detecting neonicotinoid pesticide content (claimed). ADVANTAGE - The graphene electrode: improves the stability of electrochemical detection. DETAILED DESCRIPTION - Preparing chitosan oligosaccharide three dimensional graphene electrode comprises (i) adding chitosan oligosaccharide into graphene oxide aqueous solution, mixing uniformly, incubating in the reactor at 120-180 degrees C for 6-24 hours to obtain chitooligosaccharides - three-dimensional graphene hydrogel, where the ratio of the mass of the chitosan oligosaccharide and the volume of the graphene oxide aqueous solution is 0.005-0.015 g: 10 ml and the concentration of graphene oxide in the graphene oxide aqueous solution is 2-5 mg/ml, (ii) taking out the chitosan oligosaccharide-three-dimensional graphene hydrogel obtained in the step (i), placing at -80 degrees C and freezing for 6-24 hours, placing the frozen chitooligosaccharides - three-dimensional graphene hydrogel in a freeze-dryer, freeze-drying at -40 degrees C to -60 degrees C for 36-48 hours to obtain chitosan oligosaccharide - three-dimensional graphene airgel, (iii) dicing the chitosan oligosaccharide-three-dimensional graphene airgel obtained in the step (ii) to obtain airgel block, taking copper wire, applying one end of the copper wire adhesive silver gel airgel block and exposing silver plastic with insulating silicone sealing insulation to obtain chitosan oligosaccharide-three-dimensional graphene electrode. An INDEPENDENT CLAIM is also included for use of chitosan oligosaccharide - three-dimensional graphene electrode, comprising (i) placing the chitooligosaccharides - three-dimensional graphene electrode in a blank phosphate buffer pH 7.2 solution cyclic voltammetry scan to CV curve stability, (ii) taking concentrations of nicotine pesticides are 1.0 mu mol/l, 5.0 mu mol/l, 20 mu mol/l, 40 mu mol/l and 50 mu mol/l, neonicotinoid pesticides pH is 7.2 by uding phosphate buffer solution, placing the chitooligosaccharides-three-dimensional graphene electrode, sequentially placing in a nicotine pesticide concentration of 1 mu mol/l, 5 mu mol/l, 20 mmol/l, 40 mu mol/l and 50 mu mol/l, neonicotinoid pesticides pH is 7.2 by using phosphate buffer solution, incubating at room temperature for 8-12 minutes, performing the electrochemical tests at scan range of -0.2 V to -1.4 V and scan rate is 0.05 V/s, recording reduction peak current value and the horizontal coordinate of neonicotinoid pesticide content, the vertical axis is the reduction peak current value to obtain standard curve and standard equation, (iii) determining of neonicotinoid pesticide content in test solution content: placing the chitosan oligosaccharide - three-dimensional graphene electrode in the test solution, performing electrochemical tests at scan range of -0.2 V to -1.4 V and scan rate is 0.05 V/s, determining reduction peak current value and calculating the neonicotinoid pesticide content in the test solution with the standard equation obtained in step (ii).