▎ 摘　　要

NOVELTY - Preparing medical composite collagen sponge dressing involves using electrostatic spray method to load composite gel microspheres in bacterial cellulose / graphene oxide / collagen sponge, and has rough and rough structure. The method for preparing the bacterial cellulose / graphene oxide / collagen sponge includes cleaning and removing impurities from the fish scales, adding acetic acid solution to the fish scale powder, stirring for 12-24 hours, filtering to obtain fish scale filter and filtrate, washing the fish scale filter with acetic acid solution 1-3 times. The liquid is transferred into the filtrate together and used later. The washed fish scale filter is taken, heated to 90 degrees C, sterilized for 5-10 minutes, and adding a mixture of acetic acid solution and pepsin solution to it after cooling. USE - Method for preparing medical composite collagen sponge dressing. ADVANTAGE - The method enables to prepare medical composite collagen sponge dressing, where no purification of collagen is required, and the compound sponge contains fish scale collagen, bacterial protein and nutrients of the culture medium, compound sponge has the ability to quickly stop bleeding, provides sufficient nutrients for wound healing, and promotes wound healing. DETAILED DESCRIPTION - After reacting at 36-37 degrees C for 24-36 h, ammonium bicarbonate solution is added to adjust the pH of the system to 5.0-7.0, and the supernatant is obtained by centrifugation to obtain a collagen proteolytic solution, inoculating activated bacteria with the ability to produce bacterial cellulose into the fermentation broth. After stirring and fermenting for 5-6 days, adding graphene oxide aqueous dispersion to the fermentation broth. After stirring at 400-500rpm for 12-24h, adding vegetarian meat powder, stirring well, and leaveing to age for 10-12h to obtain a paste-like product. After slicing the product in a polylysine solution at 2-4 degrees C for 24-36 h, it is dried in a freeze dryer to obtain bacterial cellulose / graphene oxide / collagen sponge.