▎ 摘　　要

NOVELTY - Preparation of molecularly imprinted composite material involves dissolving graphene oxide in deionized water, adding 1-ethyl-3-(3-(dimethylamino)propyl) carbonyl diimine hydrochloride and N-hydroxysuccinimide, stirring, adding 3-aminophenylboronic acid monohydrate, washing, filtering and drying to get boronate functionalized graphene oxide; and dispersing boronate functionalized graphene oxide in polybutylene succinate containing template glycoprotein buffer solution, adding e.g. acrylamide, N,N-methylene-bis-acrylamide, and N,N,N',N'-tetramethyl-diethylamine, crosslinking and eluting. USE - Method for preparing molecularly imprinted composite material used for enriching and separating glycoprotein (claimed). ADVANTAGE - The material has good affinity of molecular imprinting and special effects, good specificity, identification ability, high capacity, strong anti-interference ability and fast elution speed. It can adjust boron affinity interaction strength to meet their specific recognition of glycoprotein, enrichment, purification, separation and application detection. DETAILED DESCRIPTION - Preparation of molecularly imprinted composite material used for enriching and separating glycoprotein comprises dissolving graphene oxide in deionized water, ultrasonic dispersing to get oxidized paraffin ink solution, adding 1-ethyl-3-(3-(dimethylamino)propyl) carbonyl diimine hydrochloride (EDC.HCl) and N-hydroxysuccinimide, stirring for 20-40 minutes, adding 3-aminophenylboronic acid monohydrate, stirring at room temperature for 18-36 hours, filtering, washing with ethanol and water, filtering, and vacuum drying to get boronate functionalized graphene oxide; and dispersing boronate functionalized graphene oxide in polybutylene succinate containing template glycoprotein buffer solution at room temperature for 6-12 hours, assembling glycoprotein in boronate functionalized graphene oxide surface, adding acrylamide and N,N-methylene-bis-acrylamide, nitrogen-oxygen, ammonium persulfate and N,N,N',N'-tetramethyl-diethylamine, crosslinking at 18-25 degrees C for 20-30 hours, centrifuging, washing, and eluting with acetic acid and sodium acetate at pH of 2.5-4.5.