▎ 摘　　要

NOVELTY - Linker of bioprobes comprises amino thiol compound, thiol-carboxylic acid compound, dithiol compound, hydroxy carboxylic acid compound or hydroxy amine compound having a carbon number of 6 or more, where when an average surface roughness (Ra) of the chip substrate is greater than 250 nm, and a coverage of the linker on the chip substrate is 40-80%. USE - The linker of bioprobes is used for immobilizing bioprobe on chip substrate of sensor. The bioprobes comprise bioprobes of enzymes, proteins, deoxyribonucleic acid (DNA), ribonucleic acid (RNA) or odontoglossum ringspot virus antibodies (ORSV antibodies) (claimed). ADVANTAGE - The linker realizes optimal carbon chain length and the coverage for substrates of various roughness, and enhances the grasping ability of an electrochemical sensor chip for a detected object. DETAILED DESCRIPTION - Linker of bioprobes comprises amino thiol compound of formula: SH-(CH)n-NH2, thiol-carboxylic acid compound of formula: SH-(CH)n-COOH, dithiol compound of formula: SH-(CH)n-SH, hydroxy carboxylic acid compound of formula: (OH)m-(CH)n-COOH or hydroxy amine compound of formula: (OH)m-(CH)n-NH2 having a carbon number of 6 or more, where when an average surface roughness (Ra) of the chip substrate is greater than 250 nm, and a coverage of the linker on the chip substrate is 40-80%. m, n = greater than 1.