▎ 摘　　要

NOVELTY - A molecularly imprinted electrochemical sensor preparing method involves selecting glassy carbon electrode and coating graphene to obtain graphene (GR)/glassy carbon electrode (GCE). The electrode is dipped in ionic liquid (IL). The obtained electrode is immersed in oxygen, bovine hemoglobin (BHb), pyrrole monomer phosphate buffer and dried to obtain BHb/molecular imprinting polymers (MIPs)/IL/GR/GCE, followed by washing the template molecule to obtain MIPs/IL/GR /GCE, connecting obtained electrode as working electrode, reference electrode and counter electrode to form electrochemical sensors. USE - Method for preparing a molecularly imprinted electrochemical sensor that is utilized in detection of bovine hemoglobin. ADVANTAGE - The method enables preparing molecularly imprinted electrochemical sensor with excellent sensitivity, high selectivity and in fast manner. DETAILED DESCRIPTION - A molecularly imprinted electrochemical sensor preparing method involves selecting a glassy carbon electrode and surface treating the electrode. The good dispersion of graphene is coated on glassy carbon electrode surface. The coated electrode is placed in infrared lamp and dried to obtain graphene glassy carbon electrode. The obtained electrode is dipped in ionic liquid to obtain ionic liquid graphene glassy carbon electrode. The obtained mixture is immersed in oxygen, 1 g/l bovine hemoglobin, 1.0x 103-2.0x 102 mol/l pyrrole monomer phosphate buffer at 0.2-1.2 V with speed of 80-120 mV and dried to obtain bovine haemoglobin/molecular imprinting polymers/ionic liquid/graphene/glassy carbon electrode, followed by washing the template molecule using eluent to obtain molecular imprinting polymers/ionic liquid/graphene/glassy carbon electrode, connecting molecular imprinting polymers/ionic liquid/graphene/glassy carbon electrode as working electrode, reference electrode and counter electrode in an electrochemical workstation to form molecularly imprinted electrochemical sensors.