▎ 摘　　要

NOVELTY - Preparing an injectable hydrogel involves dissolving 3.6-4.4 mg of 4-carboxyphenylporphyrin (TCPP) in 4-8 ml of N,N-dimethylformamide (DMF) and absolute ethanol with a volume ratio of 3:1 until TCPP is completely dissolved. The 3.0-3.6 mg copper nitrate trihydrate (CuNO3.3H2O), 10 mg polyvinylpyrrolidone, 1.0 molar trifluoroacetic acid dissolved in N,N-dimethylformamide, then mixed and stirred for 10-20 minutes. The dopamine-reduced graphene oxide dispersion liquid added dropwise to the TCPP mixture, mixed and stirred for 10-20 minutes, then dropping is achieved through a syringe at a rate of 0.2-0.4 ml/minute. The pGO-TCPP mixture is added to the copper solution, mixed and stirred for 10-15 minutes, and the addition is realized through a syringe at a rate of 0.5-1 ml/minute, and dispersed uniformly, the dispersion is by ultrasonic dispersion, and the time is 20-30 minutes. USE - Method for preparing an injectable hydrogel suitable for diabetic wound repair. ADVANTAGE - The method prepare an injectable hydrogel with controllable antibacterial, anti-inflammatory, conductive and angiogenesis, which is suitable for oxidative stress caused by excessive ROS in diabetic wound healing, Inflammation, fragile angiogenesis, poor cell migration and proliferation, and bacterial infection, and promoting diabetic wound healing. DETAILED DESCRIPTION - Preparing an injectable hydrogel involves dissolving 3.6-4.4 mg of 4-carboxyphenylporphyrin (TCPP) in 4-8 ml of N,N-dimethylformamide (DMF) and absolute ethanol with a volume ratio of 3:1 until TCPP is completely dissolved. The 3.0-3.6 mg copper nitrate trihydrate (CuNO3.3H2O), 10 mg polyvinylpyrrolidone, 1.0 molar trifluoroacetic acid dissolved in N,N-dimethylformamide, then mixed and stirred for 10-20 minutes. The dopamine-reduced graphene oxide dispersion liquid added dropwise to the TCPP mixture, mixed and stirred for 10-20 minutes, then dropping is achieved through a syringe at a rate of 0.2-0.4 ml/minute. The pGO-TCPP mixture is added to the copper solution, mixed and stirred for 10-15 minutes, and the addition is realized through a syringe at a rate of 0.5-1 ml/minute, and dispersed uniformly, the dispersion is by ultrasonic dispersion, and the time is 20-30 minutes. The mixed solution is transferred to a high-temperature reaction kettle, and after the reaction is complete at 80℃ for 4-24 hours, and obtained pGO-Cu-TCPP two-dimensional nanosheets by centrifuging the mixture. The 0.4-0.6 g of chitosan is dissolved in 1-2% v/v acidic solution, pH=5.5-6.5, and stirred overnight, weighed 0.591-0.628 g of dihydrocaffeic acid and dissolved in 5-8 ml of absolute ethanol, poured into the above acidic solution, and added dropwise through a syringe at a rate of 1-2 ml/minute. The 1.25-1.5g of 1-ethyl-carbodiimide (EDC) dissolved in 20-25 ml of absolute ethanol, and poured into the above mixture, then added dropwise through a syringe at a rate of 5-8 ml/minute. The reaction mixture is mixed and stirred for 20-24 hours, then used the dialysis bag to purify the CS-DA mixture by dialyzing with reverse osmosis (RO) water for 3-5 days, and finally freeze-dried the product to obtain CS-DA. The 6-8 g of amylopectin added into 50 ml of RO water and stirred into starch milk. The 4.28-4.78 mg of sodium periodate (NaIO4) is mixed and dissolved in 100-150 ml of RO water, and after the dissolution is complete, added to the starch milk within 30 minutes. The mixture is reacted in the dark at 30-37℃ for 4-5 hours, then added 1-1.5 ml of ethylene glycol to end the reaction, dialyzed and purified the mixture for 3-5 days, and finally lyophilized the product to obtain aminomethylphosphonic acid (AMPA). The 0.6-1 g of CS-DA is weighed and dissolved in 10 ml of RO water, then weighed 1-1.5 g of AMPA and dissolved 10 ml of RO water at 85-95℃. A cross-linking agent is added to get the required injectable pGO-Cu-TCPP+CS-DA+AMPA hydrogel.