▎ 摘　　要

NOVELTY - Preparing electrochemical biosensor comprises (i) preparing steps of Prussian blue, (ii) preparing steps of Prussian blue/graphene hybrid nanoparticles, (iii) performing Uricase grafting, and (iv) preparing electrode, where the uricase grafting step is to take the hollow fiber membrane that has been light-induced surface carboxylation and wash it with absolute ethanol 2-3 times to remove surface impurities, and in a vacuum environment, allowing to stand for less than or equal to 24 hours to remove water and ethanol, immersing the hollow fiber membrane in 20-400 mu M uricase solution, soaking for 5-10 hours at 35-40 degrees C, immersing in 0.1-0.2M ethanolamine, soaking at 35-40 degrees C for 5-10 hours, blocking unreacted active sites, washing the blocked hollow fiber membrane with deionized water for less than or equal to 12 hours, taking out the cleaned hollow fiber membrane, and absorbing the residual liquid on the surface with a filter paper. USE - The method is useful for preparing electrochemical biosensor. ADVANTAGE - The method reduces the impedance of the electrode, the impedance measured by the electrochemical workstation is 70.34, and improves the electrochemical performance is significantly, response current of the sensor to the concentration of uric acid has been significantly and the correlation square coefficient between the measured sensor oxidation peak current and the scanning speed is 0.9803-0.9943.