▎ 摘　　要

(CN212560268-U) NOVELTY - The utility model belongs to the technical field of virus detection, relating to a double-layer microfluidic chip for detecting virus nucleic acid. double-layer micro-fluidic chip, the chip comprises a sampling layer and a detection layer, the sampling layer is provided with at least two sampling ports; the detection layer is provided with a detection cavity and a sample outlet; the detection cavity is connected with the sample feeding port through a sample feeding micro-flow passage; the sample outlet is connected with the detecting cavity through the sample outlet micro-flow channel. The utility model uses the closed double-layer microfluidic chip detection system; different articles or reagents are added from different sample adding ports, which can reduce the pollution interference caused by the external nucleic acid. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) kit comprising the double-layer microfluidic chip, a detection solution containing RNA-dependent RNA polymerase (RdRP) gene fluorescent probe and envelope protein (E) gene fluorescent probe, coronavirus standard solution, nanomaterial solution and cationic solution; and (2) method for detecting the novel coronavirus involves (a) injecting the detection solution containing RdRP gene fluorescent probe and E gene fluorescent probe, nanomaterial solution and cationic solution from one of the injection ports into the detection cavity, adsorbing the DNA probe by the nanomaterial and quenching the fluorescence, (b) injecting the solution to be tested for coronavirus from another injection port to make it enter the detection cavity, (c) after incubating the chip at room temperature to 60 degrees C for 5-40 minutes, detecting the fluorescence signal in the detection chamber and recording the fluorescence value, (d) calculating the standard linear regression equation based on the gradient concentration and fluorescence intensity of the virus standard solution and (e) substituting the measured fluorescence intensity of the test solution into the standard linear regression equation to calculate the concentration of the novel coronavirus gene in the test solution, or (a) injecting the detection solution containing the RdRP gene fluorescent probe and the E gene fluorescent probe and the coronavirus test solution from different injection ports, allowing two solutions to enter detection chamber and incubating at room temperature to 60 degrees C for 5-40 minutes, (b) injecting the nanomaterial solution from the injection port to make it enter the detection cavity, (c) detecting the fluorescence signal in the detection chamber and recording the fluorescence value, (d) calculating the standard linear regression equation based on the gradient concentration and fluorescence intensity of the virus standard solution, (e) substituting the measured fluorescence intensity of the test solution into the standard linear regression equation to calculate the concentration of the novel coronavirus gene in the test solution. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view of double-layer microfluidic chip for detecting nucleic acid. Sample application layer (1) Detection layer (2) First sample application port (3) Second sample application port (5) Third sample application port (7) Detection cavity (9) Sample outlet microchannel (11) Sample outlet (12)