▎ 摘　　要

NOVELTY - Use of graphene doped as a sensing material, involves (i) placing glassy carbon electrode in quartz tube, heating quartz tube, heat-preserving, introducing carbon source gas and hydrogen during the heat preservation process, changing to argon gas and cooling to obtain graphene modified electrode, (ii) dripping glucose oxidase phosphate buffer solution to graphene modified electrode, soaking glassy carbon electrode in phosphate buffer solution to remove glucose oxidase that is not firmly bound to graphene oxide, and drying to obtain sensor electrode, and (iii) constituting three-electrode system of sensor electrode prepared in step (ii), saturated calomel electrode and platinum sheet electrode as sensor, adding glucose to phosphate buffer solution to form electrolyte, performing cyclic voltammetry scan on electrochemical workstation, so that redox peak current on cyclic voltammetry curve changes with concentration of glucose, and realizing test of glucose concentration, is claimed. USE - The graphene doped is used as sensing material. ADVANTAGE - The graphene and graphene oxide composite material and glucose oxidase composite modified electrode has excellent glucose detection performance, and exhibits high sensitivity, wide detection range and excellent anti-interference property. DETAILED DESCRIPTION - Use of graphene doped as a sensing material, involves (i) placing glassy carbon electrode in a quartz tube, heating the quartz tube at 600-1200 degrees C under the protection of argon, heat-preserving for 20 minutes, introducing carbon source gas and hydrogen during the heat preservation process, after the reaction is completed, changing to argon gas and cooling to room temperature to obtain a graphene modified electrode, (ii) dripping glucose oxidase phosphate buffer solution to the graphene modified electrode obtained in step (i), soaking the glassy carbon electrode dried at room temperature in a phosphate buffer solution to remove the glucose oxidase that is not firmly bound to the graphene oxide, and drying at room temperature to obtain a sensor electrode, and (iii) constituting a three-electrode system of the sensor electrode prepared in step (ii), saturated calomel electrode and platinum sheet electrode as a sensor, adding glucose to 0.05-0.5 mol/l phosphate buffer solution to form electrolyte, performing cyclic voltammetry scan on electrochemical workstation in voltage of 0.6 to -0.6 V, so that the redox peak current on the cyclic voltammetry curve changes regularly with the concentration of glucose, and realizing the test of the glucose concentration, is claimed.