▎ 摘　　要

NOVELTY - Three-dimensional graphene interface microfluidic chip comprises sub-chips which are arranged in a test flow channel (21), where two ends of the test flow channel are respectively connected to a liquid reservoir (22), two liquid reservoirs and a test flow channel constitute H-shaped runner. The sub chips are arranged with cell electrodes, where cell electrodes (10) are arranged symmetrically with axis of the sub chips. One side of working electrode is set on the one hand and a counter electrode and a cell electrode in a direction. USE - Three-dimensional graphene interface microfluidic chip. ADVANTAGE - Three-dimensional graphene interface microfluidic chip has good biocompatibility, mild preparation conditions, simple and easy operation, controllable process parameters and twice the electrical signal strength of the single cell detected by the ordinary microfluidic chip. DETAILED DESCRIPTION - Three-dimensional graphene interface microfluidic chip comprises sub-chips which are arranged in a test flow channel (21), where two ends of the test flow channel are respectively connected to a liquid reservoir (22), two liquid reservoirs and a test flow channel constitute H-shaped runner. The sub chips are arranged with cell electrodes, where cell electrodes (10) are arranged symmetrically with axis of the sub chips. One side of working electrode is set on the one hand and a counter electrode and a cell electrode in a direction which is opposite and perpendicular to the center reference electrode (20). The cell electrodes are independent from each other, and are respectively connected to terminals arranged on the edge of the microfluidic chip through lead wires. The cell electrode comprises an on capturing groove, where capturing groove composed of a micro-electrodes with height of 30 micrometer. The gap between adjacent micro-electrode is 5 micron. The micro-electrodes are arranged in an orderly manner to form a perpendicular to the arc-like trapping surface on the similar arc shaped electrode base. The arc is divided along the minor axis of half ellipse. The electrode base comprises a gold layer and a graphene layer. The arcuate capture surface of the capture structure is covered with a graphene layer, a graphene layer is connected with gold layer. The graphene layer has micro-nano folds and textures that match the filiform pseudopodia on the cell surface. The cell electrode is a single-cell electrode or a double-cell electrode. The length of the minor axis corresponding to the arc-shaped capturing surface is 16-20 micrometer, and the length of the major axis is 32-36 micrometer for a single-cell electrode. The length of the short axis of the semi-ellipse corresponding to the arc-shaped capture surface of the cell electrode is 27-33 micrometer, and the length of the long axis is 40-45 micrometer. An INDEPENDENT CLAIM is included for a method for preparing a microfluidic chip with a three-dimensional graphene interface, which involves: (A) constructing two liquid reservoirs and two liquid reservoirs, where the reservoir is present between test flow passage to form H-shaped poly dimethylsiloxane, poly(dimethylsiloxane) flow channels and fixedly arranged with sub-chip of the cell electrode; (B) preparing cell electrode involves forming mutually independent electrode units on a glass substrate by a lift-off process, the electrode units having three-layer structure, which is Titanium/gold/Chromium in order from top to bottom; (C) washing titanium layer with a mass fraction of 1% hydrofluoric acid to obtain a complete patterned gold electrode array; (D) forming capture groove perpendicular to the electrode unit on the electrode unit by soft lithography, where the capture groove is composed of micro-electrodes with a gap of 5 micrometer between adjacent micro-electrodes; (E) arranging microelectrodes in an orderly manner to form an arc-shaped capture surface perpendicular to the electrode unit, where the arcs are semi-ellipsoids divided along the minor axis, treated with oxygen plasma at 20 Watt for 1 minute; (F) soaking in 10 milliliter of 20% aqueous solution of polydiallyl dimethyl ammonium chloride (PDDA) for 20 minutes; (G) allowing to stand still in an incubator at 35 degrees C for 1 hour, spraying 300 microliter of a 1 mg/mL oxidation-reduction graphene (GO) solution by atomization after washing and drying at 40 degrees C in room temperature for 12 hours, and reducing GO; (H) annealing at 200 degrees C for 2 hours in a stream of dry nitrogen; and (I) patterning graphene film with a laser beam having an energy density of 0.8 Joule/cm2 to obtain individual cell electrodes. DESCRIPTION OF DRAWING(S) - The drawing shows schematic view of three-dimensional graphene interface microfluidic chip. Cell electrodes (10) Electrode base (11) Reference electrode (20) Test flow channel (21) Liquid reservoir (22)