▎ 摘　　要

NOVELTY - Preparing multivariate electrochemiluminescence DNA sensor comprises e.g. washing glassy carbon electrode using 1-0.05 mu m nano alumina powder, ultrasonic cleaning using distilled water and ethyl alcohol to remove adsorbed material, drying under pure nitrogen environment to obtain surface polished glassy carbon electrode, taking 10-30 mu ml aqueous graphene oxide (0.1-0.3 mg/ml), adding on glassy carbon electrode surface in dropwise manner, drying electrode surface at 20-30 degrees C to obtain thin film graphene oxide thin film, at 0-1.5 V potential, 0.1-0.5 V/secons scan rate, carrying out scanning. USE - The multivariate electrochemiluminescence DNA sensor is useful for detecting virus, preferably hepatitis B virus target DNA and hepatitis C virus target DNA.(all claimed). ADVANTAGE - The sensor is simple, stable and economical and has high sensitivity. DETAILED DESCRIPTION - Preparing multivariate electrochemiluminescence DNA sensor comprises (i) washing glassy carbon electrode using 1-0.05 mu m nano alumina powder, ultrasonic cleaning using distilled water and ethyl alcohol to remove adsorbed material, drying under pure nitrogen environment to obtain surface polished glassy carbon electrode, (ii) taking 10-30 mu ml, aqueous graphene oxide (0.1-0.3 mg/ml), adding on step (i) glassy carbon electrode surface in dropwise manner, drying electrode surface at 20-30 degrees C to obtain thin film graphene oxide thin film, which is referred as graphene oxide/glassy carbon electrode, (iii) under 0-1.5 V potential, 0.1-0.5 V/secons scan rate, 7-7.5 pH phosphate buffered saline, providing glassy carbon electrode as working electrode, platinum wire electrode as counter electrode, saturated calomel electrode as reference electrode, continuing scanning step (ii) graphene oxide/glassy carbon electrode for 20 cycles, graphene oxide undergoes electrochemical reduction to obtain graphene sheet, thus glassy carbon electrode surface obtains graphene sheet, which is referred as graphene sheet/glassy carbon electrode, (iv) taking 5-10 mu ml, 530-550 nm CdTe QDs mark hepatitis B virus capture DNA and 5-10 mu ml, 600-620 nm CdTe QDs mark hepatitis C virus capture DNA solution, dropping on step (iii) graphene sheet/glassy carbon electrode surface, drying at 20-30 degrees C, obtaining CdTe QDs marked glassy carbon electrode surface virus capture DNA/graphene sheet composite thin film, which is referred as CdTe QDs labelled virus capture DNA/graphene sheet/glassy carbon electrode, (v) soaking step (iv) CdTe QDs labelled virus capture DNA/graphene sheet/glassy carbon electrode in its complementary base Hepatitis B virus target DNA and Hepatitis C virus target DNA mixed solution, mixing at 90-95 degrees C for 5-10 minutes, cooling rapidly to 20-30 degrees C, where glassy carbon electrode surface obtains viral target DNA/CdTe QDs labeled virus capture DNA/graphene sheet composite film, which is referred as viral target DNA/CdTe QDs labeled virus capture DNA/graphene sheet/glassy carbon electrode and (vi) soaking step (v) viral target DNA/CdTe QDs labeled virus capture DNA/graphene sheet/glassy carbon electrode in virus capture DNA bases complementary Gold nanoparticles labeled Hepatitis B virus DNA probe and Gold nanoparticles labeled Hepatitis C virus DNA probe mixed solution, mixing at 90-95 degrees C for 5-10 minutes, cooling rapidly to 20-30 degrees C, where glassy carbon electrode surface obtains Gold nanoparticles labeled virus probe DNA/viral target DNA/CdTe QDs labeled virus capture DNA/graphene sheet composite film, which is referred as Gold nanoparticles labeled virus DNA probe/target virus DNA/CdTe QDs labeled virus capture DNA/graphene sheet/glassy carbon electrode.