▎ 摘　　要

NOVELTY - A preparation method of micro-nano three-dimensional printing conductive biological support comprises designing biological support model, drawing two-dimensional model of support by software, converting model file into printing path file, importing the path file into the printing software, and performing (S1) selecting an insulating hard material as the substrate, carrying out pretreatment, (S2) selecting biodegradable polymers, using electric field-driven spray deposition micro-nano three-dimensional printing technology to print biological support on the pretreated substrate, forming a Taylor cone at the outlet to generate a cone jet, and the printing material is ejected downward, and printing a biological support on the substrate, and (S3) selecting a conductive material solution, wrapping the conductive material on the surface of the biological support to obtain a high conductive biological support with electrical conductivity. USE - The method is used for preparing micro-nano three-dimensional printing conductive biological support used as tissue engineering cell growth sites and carriers. ADVANTAGE - The biological support after surface coating treatment has low resistivity, high conductivity, and good for the electric signal to be transmitted through the biological support. The coating avoids the problem that the filler method needs to be pre-mixed before the molten material, and it is easy to ensure the uniformity of the mixing material. DETAILED DESCRIPTION - A preparation method of micro-nano three-dimensional printing conductive biological support comprises designing biological support structure model, drawing the two-dimensional model of support by software, converting model file into printing path file, importing the path file into the printing software, and performing (S1) selecting an insulating hard material as the substrate, carrying out pretreatment on the substrate to reduce the surface energy of the substrate, and set aside, (S2) selecting biodegradable polymers as printing materials, using electric field-driven spray deposition micro-nano three-dimensional printing technology to print biological support on the pretreated substrate, forming a Taylor cone at the outlet to generate a cone jet, and the printing material is ejected downward from the nozzle under the action of an electric field, and printing a biological support with a preset pattern on the substrate, and (S3) selecting a conductive material solution with a polarity opposite to that of the printing voltage to coat the surface of the support, and through the electrostatic attraction between the charged biological support and the conductive material, wrapping the conductive material on the surface of the biological support to obtain a high conductive biological support with electrical conductivity. An INDEPENDENT CLAIM is included for a micro-nano three-dimensional printing conductive biological support comprising the minimum surface resistance value which can reach 14.48 Ω.