▎ 摘　　要

NOVELTY - The device has a nanopore membrane (100) in which the nanopores are formed. A first reservoir (200) is provided on one side of the nanopore membrane, and in which a sample-loaded solution is accommodated. A second reservoir (300) is provided on the other side of the nanopore membrane, and in which a solution in which the sample passing through the nanopore is loaded is accommodated. A pair of first and second electrodes (410,420) are provided in the first reservoir and the second reservoir, respectively. A nano-layered membrane (500) is coupled to one of one side or other side of the nanopore membrane. The nanoflakes (510) are spaced apart from each other and arranged in a nanolayered structure. The lower portion of the nanolayered membrane forms an empty space so that the nanopores are not shielded by the nanolayered membrane. USE - Nanopore device for detecting sample passing through nanopores, and for controlling passage of very small amounts of ions. ADVANTAGE - The device has the effect of effectively detecting the sample because the speed of the sample to pass through the nanopore can be controlled by bonding the nanolayer membrane to the nanopore membrane. The speed of passing materials such as DNA or biomaterials can be sufficiently slowed down before passing through the nanopores by using a lamella-type stacked structure stacked in a layered structure with interlayer spacing materials as needed. The DNA or biomaterials move from a coiled form to a stretched form when DNA or biomaterials pass through a nano-sized layered channel, which is easy to detect when entering the nanopore. The layered structure of lamella using flakes of nanomaterials is simple to manufacture, and easy to combine with existing nanopores. The passage speed of the sample is controlled by changing the layer spacing, thickness, size of flakes. DESCRIPTION OF DRAWING(S) - The drawing shows a cross-sectional view showing a nano-pore device to which a nano-layered structure film is coupled. Nanopore membrane (100) First reservoir (200) Second reservoir (300) First electrode (410) Second electrode (420) Nano-layered membrane (500) Nanoflakes (510)