▎ 摘　　要

NOVELTY - Method for optimizing loop-mediated isothermal amplification reaction involves (a) preparing graphene quantum dots and carbon nanotubes, (b) purifying the carbon nanotubes, (c) dispersing the mixture, heating, and preparing amplified product, (d) selecting the to-be-amplified product, checking the characteristics, designing the amplification primers, setting the volume of the amplified product and the amplification conditions, and establishing the loop-mediated isothermal amplification reaction system, and (e) adding the amplified product of the optimized loop-mediated isothermal amplification reaction to the established loop-mediated isothermal amplification reaction system, reacting, adding the optimized amplified product to the loop-mediated isothermal amplification reaction system again, amplifying nucleic acid, preparing a nucleic acid reaction system, and completing the optimization of the loop-mediated isothermal amplification reaction. USE - The method is useful for optimizing loop-mediated isothermal amplification reaction. ADVANTAGE - The method is simple, has good optimization effect, and is suitable for use in molecular detection. DETAILED DESCRIPTION - Method for optimizing loop-mediated isothermal amplification reaction involves (a) preparing graphene quantum dots and carbon nanotubes respectively by weighing and using 8-12 parts mass graphene quantum dots, and 2-3 parts mass carbon nanotubes as raw materials for preparing the amplified product of the optimized ring-mediated isothermal amplification reaction, and purifying the graphene quantum dots by (i) mixing the initial graphene quantum dot solution at room temperature with molecular sieves in a certain ratio to obtain reactants, (ii) centrifuging the reactant to obtain a graphene quantum dot-molecular sieve containing graphene quantum dots, and (iii) adding a desorbent to the graphene quantum dot-molecular sieve, and desorbing the graphene quantum dot under the condition of 70-100 degrees C, (b) purifying the carbon nanotubes, preliminarily pulverizing the purified graphene quantum dots and carbon nanotube powders, ball-milling the graphene quantum dots after preliminary pulverization to obtain graphene quantum dot powder with a particle size of not less than 800 nm, ball-milling the pulverized carbon nanotubes to obtain carbon nanotube powder with a particle size of less than 200 mu m, and collecting and mixing graphene quantum dot powder and carbon nanotube powder obtained after ball milling to obtain a mixture, (c) dispersing the mixture obtained after the treatment in deionized water, heating and separating out, and preparing the amplified product of the optimized ring-mediated isothermal amplification reaction to obtain the amplified product of the optimized ring-mediated isothermal amplification reaction, (d) selecting the to-be-amplified product according to the amplification requirements, checking the characteristics of the to-be-amplified product, designing the amplification primers based on the information of the to-be-amplified product, setting the volume of the amplified product and the amplification conditions according to the acquired information of the amplified product, and then establishing the loop-mediated isothermal amplification reaction system, and (e) adding the amplified product of the optimized loop-mediated isothermal amplification reaction to the established loop-mediated isothermal amplification reaction system, performing the initial reaction of the loop-mediated isothermal amplification reaction system, after the amplification reaction starts, adding the amplified product optimized for the loop-mediated isothermal amplification reaction to the loop-mediated isothermal amplification reaction system again, performing nucleic acid amplification, preparing a nucleic acid reaction system, and completing the optimization of the loop-mediated isothermal amplification reaction. The carbon nanotubes are purified by adding a parallel magnetic field along the carbon nanotube growth direction to the carbon nanotube growth area to make the magnetic field interact with the magnetic nanoparticles, subjecting the magnetic nanoparticles to the force of the magnetic field in the growth direction, and using magnetic nano-catalyst particles to drive the directional growth of carbon nanotubes under the force of a magnetic field to obtain purified carbon nanotubes. The amplified product for the optimized loop-mediated isothermal amplification reaction is prepared by (i) placing the mixture obtained after treatment in deionized water for ultrasonic dispersion, (ii) after the solid matter in the water is evenly dispersed, heating the aqueous solution, (iii) after heating, fully stirring the aqueous solution and standing for 1-2 hours, and (iv) rapidly cooling the aqueous solution after standing to obtain the amplified product of the optimized loop-mediated isothermal amplification reaction.