▎ 摘　　要

NOVELTY - Preparation of copper-based super-resolution fluorescence-imaging sample holder involves forming graphene layer on a copper foil, irradiating ion beams, dripping reduced amount of polymethylmethacrylate on surface of graphene layer to solidify graphene layer, immersing obtained composite in ferric chloride solution, washing using deionized water, drying, observing using microscope whether 5 mu m-copper is fully dissolved, and repeating process until 5 mu m-copper is fully dissolved to obtain copper-based graphene layer. USE - Preparation of copper-based super-resolution fluorescence-imaging sample holder (claimed). ADVANTAGE - The method efficiently and economically provides copper-based super-resolution fluorescence-imaging sample holder which improves image contrast, reduces unwanted background scattering, reduces sample fluorescence quenching, and reduces damage to living tissue. DETAILED DESCRIPTION - Preparation of copper-based super-resolution fluorescence-imaging sample holder involves forming graphene layer on a copper foil having thickness of 25 mm and purity of 99.98%, irradiating ion beams to depth of 20 mu m and aperture radius of 2 mu m, dripping reduced amount of polymethylmethacrylate on the surface of graphene layer to solidify the graphene layer, immersing obtained composite in a ferric chloride solution for 10 minutes such that the composite surface is fully contacted with the solution, washing using deionized water, drying, observing using microscope whether 5 mu m-copper is fully dissolved, repeating process until 5 mu m-copper is fully dissolved and obtaining copper-based graphene layer, and then immersing obtained copper-based graphene layer in acetone solution to remove polymethylmethacrylate from surface.