▎ 摘 要
NOVELTY - Preparing folate-porous platinum-graphene oxide composite nanomaterial comprises: (a) preparing graphene oxide aqueous solution at 0.5 mg/ml, adding polyallylamine hydrochloride and potassium hydroxide, uniformly mixing at 70 degrees C, water bath heating for 24 hours, collecting solution, and centrifugally washing; (b) using 1-3-(3 amino propyl)-carbodiimide (EDC) and N-hydroxyl succinimide (NHS) as cross-linking agent; and (c) taking folic acid functionalized graphene oxide water solution with the chlorine molybdenum acid solution with concentration of 7.9 mmol/L to the volume ratio of 10:1. USE - The method is useful for preparing folate-porous platinum-graphene oxide composite nanomaterial (claimed). The nanomaterial is useful for detecting tumor cells, preferably human breast cancer MCF-7 cells. ADVANTAGE - The nanomaterial can be used for rapid and sensitive detection of tumor cells. DETAILED DESCRIPTION - Preparing folate-porous platinum-graphene oxide composite nanomaterial comprises: (a) preparing graphene oxide aqueous solution at 0.5 mg/ml, adding polyallylamine hydrochloride and potassium hydroxide, uniformly mixing at 70 degrees C, water bath heating for 24 hours, collecting solution, centrifugally washing, and removing allylamine hydrochloride and potassium hydroxide; (b) using 1-3-(3 amino propyl)-carbodiimide (EDC) and N-hydroxyl succinimide (NHS) as cross-linking agent, reacting for 24 hours, collecting the solution of folic acid, functionalized centrifugal washing to remove excessive cross-linking agent and acid to obtain graphene oxide water solution; and (c) taking folic acid functionalized graphene oxide water solution with the chlorine molybdenum acid solution with concentration of 7.9 mmol/L to the volume ratio of 10:1, water bath heating at 80 degrees C for 24 hours to obtain the folate-porous platinum-graphene oxide water solution, and storing at 4 degrees C.