▎ 摘　　要

NOVELTY - Preparing defective graphene surface-enhanced Raman scattering (SERS) substrate comprises (1) adding silver nitrate and polyvinylpyrrolidone to dimethylformamide, reacting for 6-8 hours at temperature of 150-180 degrees C, and obtaining silver nanoparticles after cooling, (2) washing graphene with aqueous solution until it is neutral after the graphene is carboxylated, ultrasonically processing in nitric acid solution for 1-24 hours, washing, and centrifuging and drying to obtain uniformly distributed defective grapheme, (3) ultrasonically dispersing thedefect graphene in aqueous solution and adding to ethanol solution of silver nanoparticles, stirring at room temperature for 6-48 hours, and centrifuging to obtain defect graphene/silver compound material with uniformly distributed silver nanoparticles, and (4) modifying defective graphene/silver compound material with silane reagent, and adding template molecule, functional monomer, cross-linking agent, initiator to acetonitrile. USE - The method is useful for preparing defective graphene surface-enhanced Raman scattering (SERS) substrate. ADVANTAGE - The method defects of the graphene are used to anchor the gold nanoparticles to make their dispersion more uniform and sites combined with molecularly imprinted polymers to further improve the adsorption performance of the SERS substrate. The substrate has high sensitivity, selectivity and reusability. DETAILED DESCRIPTION - Preparing defective graphene surface-enhanced Raman scattering (SERS) substrate comprises (1) adding silver nitrate and polyvinylpyrrolidone to dimethylformamide, reacting for 6-8 hours at temperature of 150-180 degrees C, and obtaining silver nanoparticles after cooling, (2) washing graphene with aqueous solution until it is neutral after the graphene is carboxylated, ultrasonically processing in nitric acid solution for 1-24 hours, washing, and centrifuging and drying to obtain uniformly distributed defective grapheme, (3) ultrasonically dispersing thedefect graphene in aqueous solution and adding to ethanol solution of silver nanoparticles, stirring at room temperature for 6-48 hours, and centrifuging to obtain defect graphene/silver compound material with uniformly distributed silver nanoparticles, and (4) modifying defective graphene/silver compound material with silane reagent, adding template molecule, functional monomer, cross-linking agent, initiator to acetonitrile, reacting at certain temperature for certain time under nitrogen, coating surface of the defective graphene/silver with imprinted polymer film, washing template molecules away by washing liquid, and vacuum drying resulting product.