▎ 摘　　要

NOVELTY - Inhibiting microbial activity involves administering effective amount of reduced graphene oxide nano-particles to site of microbial activity, where reduced graphene oxide nano-particles are produced by green synthesis of reduced graphene oxide, mixing carbon source in acid while stirring to obtain solution. The first oxidant is added into solution to oxidize carbon source to obtain suspension. The suspension is stirred, and temperature of suspension is maintained at 35 degrees C. The Nigella sativa seed extract is added to suspension and temperature of the suspension is raised to 60 degrees C. USE - Method for inhibiting microbial activity (claimed). ADVANTAGE - The method enables to inhibit microbial activity that is convenient, does not use of hazardous chemical and organic solvents, and in cost effective manner. DETAILED DESCRIPTION - Inhibiting microbial activity involves administering effective amount of reduced graphene oxide nano-particles to site of microbial activity, where reduced graphene oxide nano-particles are produced by green synthesis of reduced graphene oxide, mixing carbon source in acid while stirring to obtain solution. The first oxidant is added into solution to oxidize carbon source to obtain suspension. The suspension is stirred, and temperature of suspension is maintained at 35 degrees C. The Nigella sativa seed extract is added to suspension and temperature of the suspension is raised to 60 degrees C. The hydrogen peroxide is added to suspension, and resulting reduced graphene oxide nanoparticles is isolated by centrifugation. The diamter of graphene oxide nanoparticles is 5-200 nanometer.