▎ 摘　　要

NOVELTY - Detecting glucose by non-enzymatic electrochemical biosensing method comprises e.g. (i) dispersing graphene oxide on surface of working electrode of screen-printed carbon electrode, (ii) taking gold nanoparticles, dispersing gold nanoparticles in container, (iii) adding gold nanoparticle-concanavalin A nanoprobe and glucose standard solution into sensor, and (iv) taking clinical serum samples and performing 5 parallel experiments on sample, adding gold nanoparticle-concanavalin A nano-probe and sample solution into sensor, treating standard solution to detect serum samples of glucose content. USE - The method is useful for detecting glucose by non-enzymatic electrochemical biosensing method (claimed). ADVANTAGE - The method is simple to operate and product has high sensitivity and low cost. DETAILED DESCRIPTION - Detecting glucose by non-enzymatic electrochemical biosensing method comprises (i) dispersing 1.8 mu l 1 mg/ml graphene oxide onto the surface of the working electrode of screen-printed carbon electrode, drying at room temperature and transferring to a concentration of 50 mM tris-hydrochloride at pH of 6.5 using potentiostatic electrochemical reduction method at potential of -1.2 V, where scanning rate is 100 mV/s, and time is 600 s, reducing graphene oxide into graphene, washing with water at room temperature, drying to obtain reduced graphene modified electrode, adding reduced graphene modified electrode into 2 mu l 3 mu M phenyl-dextran in 10 mM pH of 7.4 using tris-hydrochloride solution, reacting at 37 degrees C for 2 hours, washing and drying, placing in dry 4 degrees C environment, (ii) taking 1 ml diameter of 13 nm gold nanoparticles, discarding supernatant by centrifugation, dispersing gold nanoparticles in container containing 1 ml 5 mM mixed solution of pH 7 using tris-HCl and 0.5% Tween-20 (RTM: Polyoxyethylene sorbitan monolaurate), adding 500 mu l 3-mercaptopropionic acid solution having concentration of 1 mM, rotating reaction mixture for 2 hours, and then centrifugating resulting solution, washing twice with 10 mM tris-HCl solution at pH 7, adding 100 l 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride at concentration of 3 mg/ml, adding 60 mu l concanavalin A at concentration of 1 mg/ml and mixture of 100 mu l of 3 mg/ml bovine serum albumin in rotary mixer at room temperature for 1 hour, then centrifugating product by freezing, washing twice with 10 mM tris-HCl solution at pH 7, dispersing in 500 mu l tris-HCl solution containing 2 mM calcium ions and 2 mM manganese ions at a concentration of 10 mM, and storing at 4 degrees C, (iii) adding 20 mu l gold nanoparticle-concanavalin A nanoprobe and 10 mu l glucose standard solution into prepared sensor surface, and incubating for 30 minutes at 37 degrees C using 50 mM tris-HCl/Tween-20 (RTM: Polyoxyethylene sorbitan monolaurate), solution and cleaning, drying, adding 30 l 0.1 mol/l hydrochloride solution, performing pre-oxidation at a constant potential of 1.3 V for 40 s, recording current response by differential pulse voltammetry in range of 0.5-0 V, and testing standard solution of glucose content, and (iv) taking clinical serum samples and performing 5 parallel experiments on each sample, adding 20 mu l gold nanoparticle-concanavalin A nano-probe and 10 mu l sample solution into prepared sensor surface, treating above standard solution to detect serum samples of glucose content.