▎ 摘　　要

NOVELTY - Kit comprises functionalized oxide graphene, fluorescein isothiocyanate (FITC)-labeled single chain DNA having sequence of 5'-tgacctgccattgag-3', a reagent, formaldehyde, escin and a phosphate buffer saline, where the concentration of the above components are given in table of specification. USE - The kit is useful for detecting L-type promyelocytic leukemia/retinoic acid receptor (PML/RAR) alpha fusion gene (claimed) and diagnosing acute promyelocytic leukemia. ADVANTAGE - The kit simply, rapidly and accurately detect existence of L-type PML/RAR alpha fusion gene cell with high sensitivity and specificity, APL diagnosis and minimal residue monitoring, and the kit can realize recurrence prediction at early stage and clinical treatment guidance. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for detecting L-type PML/RAR alpha fusion gene comprises (1) culturing the cell to be detected in a RPM1-1640 culture medium containing 10% of fetal calf serum, at 37 degrees C under the presence of 5% of carbon dioxide, changing the half-amount liquid for 2-3 days, (2) mixing the obtained cells in a functional solution containing graphene oxide, FITC-labeled single-stranded DNA and a reagent mixture to obtain incubating solution A, where the FITC-labeled single-stranded DNA has sequence of 5'-gacctgccattgag-3', (3) introducing the pre-processed cell into a centrifugal tube and then centrifuging at room temperature and speed of 1000 revolutions per minute (rpm) for 5 minutes to remove the supernatant, adding 100 mu l of formaldehyde in the centrifugal tube at room temperature for 10-15 minutes and then adding 4 ml of phosphate buffered saline (PBS) buffering liquid and then mixing at room temperature, centrifuging the mixture at 1000 rpm for 5 minutes, removing the supernatant, and (4) adding obtained breeding solution obtained in step (2) breeding with the product obtained in step (3) in a centrifuge tube and then mixing at temperature of 37 degrees C for 1 hour and centrifuging at speed of 1000 rpm for 5 minutes, removing the supernatant adding 4 ml of PBS buffer solution in the centrifugal tube at room temperature and then centrifuging at 1000 rpm for 5 minutes, removing the supernatant and then analyzing the result.