▎ 摘　　要

NOVELTY - Detecting an advanced glycation end product comprises e.g. (i) dripping graphene-chitosan on the surface of the polished and cleaned glassy carbon electrode, drying, coating with N( epsilon )carboxymethyl lysine solution on the surface, and drying; (ii) formulating an ethanol solution containing the advanced glycation end product; (iii) immersing electrochemical immunosensor in a phosphate buffer solution, washing electrochemical immunosensor with a phosphate buffer solution, placing in ferrocene acetonitrile solution for differential pulse voltammetry scanning and plotting logarithmic log C of the concentration of the late glycation end product in the standard solution against the response current change value Delta I before; (iv) configuring ethanol solution containing the sample to be tested, incubating electrochemical immunosensor, scanning differential pulse voltammetric in the same manner, recording and substituting into linear regression equation. USE - The method is useful for detecting an advanced glycation end product. ADVANTAGE - The method solves the problem that the current advanced glycation end product detection method can only detect a single substance, produces has high sensitivity (detection limit is between 0.06-0.1 ng/ml) and wide linearity range is (0.1-1000 ng/ml), is easy to operate and economical. DETAILED DESCRIPTION - Detecting an advanced glycation end product comprises (i) dripping graphene-chitosan on the surface of the polished and cleaned glassy carbon electrode, drying, coating with N( epsilon )carboxymethyl lysine solution on the surface, and drying to obtain the electrochemical immunosensor; (ii) formulating an ethanol solution containing the advanced glycation end product as a standard solution, and using a standard solution containing a late glycation end product of concentration 0 as a blank standard; (iii) immersing electrochemical immunosensor in a phosphate buffer solution containing a pentosidine monoclonal antibody and the standard solution, washing electrochemical immunosensor with a phosphate buffer solution after incubation, placing in a 5 mmol/liter ferrocene acetonitrile solution for differential pulse voltammetry scanning, plotting logarithmic log C of the concentration of the late glycation end product in the standard solution against the response current change value Delta I before and after the incubation Delta I/logC working curve using linear regression method to obtain Delta I-logC linear regression equation; (iv) configuring ethanol solution containing the sample to be tested, incubating electrochemical immunosensor, scanning differential pulse voltammetric in the same manner, recording response current change value Delta ix and substituting into the Delta I/logC linear regression equation to obtain concentration of the advanced glycation end product in the sample to be tested.