▎ 摘　　要

NOVELTY - Graphene photoelectric double detection biosensor comprises a glass substrate, a source electrode and a drain electrode disposed on the glass substrate, where a graphene film channel is located between the source and drain electrodes and a reaction chamber is disposed on the graphene film for containing the electrolyte and the gate. The graphene film is provided with a probe chain immobilized by pyrene butyric acid-succinimide ester, the two are combined by the pi-pi stacking effect of the graphene oxide and the probe chain. USE - Graphene photoelectric double detection biosensor. DETAILED DESCRIPTION - Graphene photoelectric double detection biosensor comprises a glass substrate, a source electrode and a drain electrode disposed on the glass substrate, where a graphene film channel is located between the source and drain electrodes and a reaction chamber is disposed on the graphene film for containing the electrolyte and the gate. The graphene film is provided with a probe chain immobilized by pyrene butyric acid-succinimide ester, the two are combined by the pi-pi stacking effect of the graphene oxide and the probe chain, and fluorescence on the probe chain can be quenched based on fluorescence resonance energy transfer. A new double conductive layer is constructed by substituting double-stranded DNA composed of graphene oxide and a probe chain by a DNA target chain and a graphene film. The change of conductivity of graphene FET and the detachment of the probe chain from the graphene oxide to the target chain make the fluorescence on the probe chain recover, which realizes the photoelectric double detection of graphene. INDEPENDENT CLAIMS are included for: (1) a method for constructing a sensor, which involves transferring the graphene film grown on the surface of the copper foil by a chemical vapor deposition method to a glass substrate with two electrodes by a wet transfer method as a channel between the two electrodes; attaching the reaction chamber made of polydimethylsiloxane to the surface of the film as a container for containing the electrolyte and the grid; and immobilizing the probe chain on the film using 1-pyrenebutyric succinimidyl ester, and adding graphene oxide, where the pi-pi stacking of graphene oxide and single-stranded probes is used to combine the two, while the fluorescence on the probe chain is quenched based on the fluorescence resonance energy transfer; and (2) a graphene photoelectric double detection system, which comprises the sensor and the detection module, where the detection module comprises an optical path detection module and a circuit detection module, the circuit detection module comprises a microprocessor, a D/A conversion unit, an A/D conversion unit and a bridge balance circuit, where the microprocessor is connected with the gate of the sensor through a digital-analog conversion unit, one end of the bridge balanced circuit measures the resistance of the graphene, and the other end through the analog/digital conversion unit connected to the microprocessor, and the optical path detection module comprises a photomultiplier tube, where the photomultiplier tube is connected with the analog/digital conversion unit and the microprocessor.