▎ 摘　　要

NOVELTY - A carrier material based on graphene oxide for a targeting anticancer drug and a gene, where folic acid, lactobionic acid and other tumor cell targeting or liver targeting molecules and part of amino groups of soluble chitosan are connected by amide bonds to prepare a conjugate, the conjugate is then connected with graphene oxide, and finally quaternization is performed by using an epoxy compound with a quaternary ammonium group, and gene molecules are loaded by the quaternization of quaternized part of the chitosan through electrostatic attraction. USE - The carrier material based on graphene oxide is useful for preparing anti-cancer drugs (claimed). ADVANTAGE - By adopting the targeting performance of targeting molecules and effects of graphene oxide of a particular size to enhance penetration and retention in tumor tissues and combining the performance of the graphene oxide for pH response control release of the loaded drug, the drug can be realized released in a tumor cell, an intelligent delivery system for the common carrier of the tumor targeting or liver targeting anticancer drug and the gene is synthesized from the perspective of synergetic medication, and a theoretical basis and a method basis are provided for combined therapy of tumor. DETAILED DESCRIPTION - A carrier material based on graphene oxide for a targeting anticancer drug and a gene, where folic acid, lactobionic acid and other tumor cell targeting or liver targeting molecules and part of amino groups of soluble chitosan are connected by amide bonds to prepare a conjugate, the conjugate is then connected with graphene oxide, and finally quaternization is performed by using an epoxy compound with a quaternary ammonium group, and gene molecules are loaded by the quaternization of quaternized part of the chitosan through electrostatic attraction; and then the anticancer drug is loaded by pi-pi conjugates, hydrogen bonds and hydrophobic effects in a non-covalent bond method, where the targeting molecules, chitosan, the graphene oxide with a quaternary ammonium group and an epoxy compound has a mass ratio of 1:1-50:0.2-20:0.1-100; preparation method is: in the presence of a dehydrating agent, the target molecule and the soluble reaction is stirred in an aqueous solution of chitosan, rotary evaporation, the concentrate over Sephadex column, targeting molecules and chitosan conjugate product was concentrated and lyophilized in the presence of a dehydrating agent and ultrasonic agitation graphene oxide reaction, separation, washing the product, and then with quaternary ammonium groups with the epoxy compound with ultrasonic agitation at 80 degrees C, after the reaction, the solid product was repeatedly washed by centrifugation, to obtain a positive charge functionalized graphene oxide, dispersed in water or in RPMI1640 medium with anticancer drugs and genetic and molecular mixing, incubation can; the antineoplastic loading of 0.05-2.0 mg/mg; gene molecules of the loading amount of 0.01-50 mu mol/g; the targeting molecule refers to a tumor-targeting or hepatic targeting of various molecules: folic acid, lactobionic acid, galactosidase, glycyrrhetinic acid or a variety of tumor cell targeting antibody and small peptides. An INDEPENDENT CLAIM is a method of preparing graphene oxide-based anticancer drugs and gene targeting carrier material, comprising: (1) targeting molecules and soluble chitosan conjugate preparation: the presence of a dehydrating agent, the targeting molecules and soluble chitosan soluble in aqueous solution, stirred at room temperature day, the system off the solvent by rotary evaporation, the concentrate over Sephadex column to remove low molecular impurities, the product is concentrated and lyophilized to obtain the two coupled product; (2) modification of the prepared coupling product in the graphene oxide, in the presence of a dehydrating agent, the targeting molecules and soluble chitosan conjugate is mixed with ultrasound graphene oxide and dissolved in water and ultrasonic bath 60 W, 1 hour, stirring at room temperature for 4 days, purifying by repeated centrifugation, ultrasonic dispersion, ultrasonic bath 60 W, 2 minutes, the cycle is washed to remove unreacted conjugate; (3) preparation of graphene oxide functionalized with 2,3-epoxypropyl trimethyl ammonium chloride soluble in aqueous solution, sodium hydroxide to adjust pH to 7-8, reacting at 80 degrees C for 24 hours, purifying by repeated centrifugation, ultrasonic dispersion, ultrasonic bath 60 W, 2 minutes cycle, and washing to remove unreacted 2,3-epoxypropyl trimethyl ammonium chloride; the targeting molecules, soluble chitosan conjugates and dehydrating agent mass ratio is 1:1-1000:0.1-10.