▎ 摘　　要

NOVELTY - Detecting chlorophyll and carotenoids content involves preparing graphene quantum dot of the light emitting wavelength. Fluorescence spectrophotometer is utilized for measuring red and blue fluorescence of the graphene quantum dot solution before adding the chlorophyll and carotenoid mixture solution to be tested with fluorescence intensity FR0, FB0 and fluorescence intensity FR1, FB1 of the fluorescence intensity FR0, FB0 followed by bringing the obtained (FR0-FR1)/FR0 into the standard relation curve of the known chlorophyll concentration and red fluorescence graphene quantum dot fluorescence intensity to obtain the concentration C1 of chlorophyll in the mixed solution to be tested, and substituting C1 into the standard relation curve, neutralizing the obtained FB0-(FB2-FB1/FB0) into the known carotenoid concentration and blue fluorescent graphene quantum dot fluorescence intensity of the standard relation curve to obtain the concentration C2 of carotenoid. USE - Method for detecting chlorophyll and carotenoids content. ADVANTAGE - The method enables detecting chlorophyll in the chloroplast, and carotenoid content with good application prospect, in simple, easy and cost effective manner. DETAILED DESCRIPTION - Detecting chlorophyll and carotenoids content involves preparing graphene quantum dot of the light emitting wavelength in the red light wave band and the blue light wave band. Fluorescence spectrophotometer is utilized for measuring red and blue fluorescence of the graphene quantum dot solution before adding the chlorophyll and carotenoid mixture solution to be tested with fluorescence intensity FR0, FB0 and fluorescence intensity FR1, FB1 of the fluorescence intensity FR0, FB0 followed by bringing the obtained (FR0-FR1)/FR0 into the standard relation curve of the known chlorophyll concentration and red fluorescence graphene quantum dot fluorescence intensity to obtain the concentration C1 of chlorophyll in the mixed solution to be tested, and substituting C1 into the standard relation curve of the known chlorophyll concentration and blue fluorescence graphene quantum dot fluorescence intensity to obtain the corresponding blue fluorescence intensity FB2, neutralizing the obtained FB0- (FB2-FB1/FB0) into the known carotenoid concentration and blue fluorescent graphene quantum dot fluorescence intensity of the standard relation curve to obtain the concentration C2 of carotenoid in the mixed solution to be tested.