▎ 摘　　要

NOVELTY - Production of palladium-nickel (PdNi) alloy/nitrogen doped graphene nanoribbon dual-amplification immunosensor involves polishing glassy carbon electrode with aluminum oxide polishing powder, cleaning with ultrapure water, placing electrode in potassium ferricyanide solution, and scanning; dropping beta-cyclodextrin graphene solution into electrode surface, and drying; dropping adamantane carboxylic acid functionalized breast cancer markers capture antibody Ab1; adding bovine serum albumin solution; adding breast cancer marker solution at different concentrations into electrode surface. USE - Method for producing PdNi alloy/nitrogen doped graphene nanoribbon dual-amplification immunosensor used for detecting breast cancer (claimed). ADVANTAGE - The immunosensor has good catalytic properties, hybrid material biocompatibility, and high catalytic efficiency, which improves sensitivity and stability of sensor. DETAILED DESCRIPTION - Production of PdNi alloy/nitrogen doped graphene nanoribbon dual-amplification immunosensor comprises: (A) washing and polishing glassy carbon electrode with 4 mm diameter with 1.0 mu m, 0.3 mu m, 0.05 mu m of aluminum oxide polishing powder, successively, cleaning with ultrapure water, placing electrode in 5 mmol/L potassium ferricyanide solution, and scanning at 0.2-0.6 V, where the peak potential is less than 110 mV; (B) dropping 6 mu L of 0.5-3 mg/mL beta-cyclodextrin graphene solution into electrode surface, and drying at room temperature; (C) dropping 6 mu L of 5-10 mu g/mL adamantane carboxylic acid functionalized breast cancer markers capture antibody Ab1, drying at 4 degrees C, and cleaning with ultrapure water; (D) adding 3 mu L 1% bovine serum albumin solution into electrode surface, drying at 4 degrees C, and cleaning with ultrapure water; (E) adding 6 mu L breast cancer marker solution at different concentrations into electrode surface, drying at 4 degrees C, and cleaning with ultrapure water; and (F) adding 4-6 mu L detection antibody incubator PdNi alloy/nitrogen doped graphene nanoribbon-Ab2 solution into electrode surface, incubating in refrigerator at 4 degrees C for 1 hour, cleaning, and drying. An INDEPENDENT CLAIM is included for preparation of antibody incubator PdNi alloy/nitrogen doped graphene nanoribbon-Ab2 solution comprising: (A) preparing 50 mg nitrogen-doped carbon nanotubes, mixing 18 mL sulfuric acid and 2 mL phosphoric acid, reacting using microwave at 50-140 degrees C for 4-30 minutes, cooling at room temperature, adding 0.15-0.5 g potassium permanganate, reacting at 60-70 degrees C for 2-10 minutes, washing, centrifuging, and vacuum drying at 35-45 degrees C to obtain nitrogen-doped graphene nanoribbons; (B) preparing detection antibody marker PdNi alloy/nitrogen doped graphene nanoribbon by taking 15 -25 mg disodium tetrachloropalladate, 20-30 mg nickel chloride hexahydrate, and 50-60 mg glutamic acid, mixing into 40 mL ethylene glycol, adjusting pH value at pH 11 using 1 mol/L sodium hydroxide solution, adding 25-35 mg nitrogen-doped graphene nanoribbons, stirring for 1-3 hours, transferring into reaction vessel, reacting at 100-200 degrees C for 2-8 hours, washing mixture, centrifuging, and vacuum drying at 35 degrees C; and (C) dispersing 1-3 mg PdNi alloy/nitrogen doped graphene nanoribbon in 1 mL ultrapure water, adding 100 mu L of 8-12 mu g/mL detection antibody solution, and 900 mu L of 1/15 mol/ L phosphate buffer solution with pH 7.4, shaking in incubator at 4 degrees C for 12 hours, centrifuging, adding 1 mu L of 1/15 mol/ L phosphate buffer solution with pH 7.4 into lower sediment, and storing final product at 4 degrees C.