▎ 摘　　要

NOVELTY - Macrophage membrane bionic photo immune nano drug delivery system comprises anthracycline antitumor antibiotic, photo-thermal response agent, cationic phospholipid, neutral phospholipids and cholesterol; the cationic liposome nanoparticle and the macrophage membrane modified on the outer surface of the cationic liposome nanoparticle form a biomimetic nanoparticle, and the biomimetic nanoparticle is loaded on a soluble micro needle to form; the anthracycline antitumor antibiotic is one of daunorubicin, doxorubicin, epirubicin, pirarubicin, mitoxantrone and idarubicin. USE - Macrophage membrane bionic photo immune nano drug delivery system for inhibiting breast cancer solid tumor growth. ADVANTAGE - The method provides combining nano technology and transdermal drug delivery technology, the antitumor agent and light response agent are accurately delivered to the tumor portion and makes up the defect that the traditional nano drug delivery system is highly dependent on the Enhanced permeability and retention (EPR) effect to induce the tumor cell immunogenic cell death in the tumor portion specific aggregation, and activates the organism immune response and effectively accelerating tumor cell death process. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for preparing of the drug delivery system, which involves: (a) ultrasonic dissolving the anthracene ring antitumor antibiotic in the organic solvent, adding triethylamine, through acid-base neutralization reaction to remove hydrochloric acid; (b) removing the hydrochloric acid of the anthracene ring antitumor antibiotics, cationic phospholipids, neutral phospholipid's and cholesterol, ultrasonic dissolving in the organic solvent, rotary evaporation to remove organic solvent, forming phospholipid double-molecular layer, adding aqueous solution containing photo-thermal response agent, hydrating and ultrasonically dispersing, filtering and granulating to obtain the cationic liposome nanoparticles; (c) taking macrophage membrane, centrifugal cracking, ultrasonic crushing, density gradient centrifuging to obtain the purified macrophage membrane; (d) taking the purified macrophage membrane, ultrasonically mixing with cationic liposome nanoparticle probe, the liposome extruder is co-extruded to obtain the bionic nanoparticle; and (e) taking the bionic nanoparticle and soluble micro-needle matrix material, ultrasonic uniformly mixing, putting the micro-needle and loading substrate material, drying and demolding to obtain the macrophage membrane bionic photo-immune nano delivery system.