• 专利标题:   Electrochemical aptamer sensor for detecting hydroxypolychlorinated biphenyl 106, comprises polydiallyl dimethyl ammonium chloride, graphene, glassy carbon electrode, nano gold particle and gene fragment, where graphene is product of graphene oxide reduced by hydrazine hydrate.
  • 专利号:   CN114660141-A
  • 发明人:   LIU A, YANG K, ZHANG G
  • 专利权人:   LUNAN PHARM CO LTD
  • 国际专利分类:   B82Y040/00, G01N027/30, G01N027/327, G01N027/48, G01N033/543
  • 专利详细信息:   CN114660141-A 24 Jun 2022 G01N-027/30 202277 Chinese
  • 申请详细信息:   CN114660141-A CN11549362 24 Dec 2020
  • 优先权号:   CN11549362

▎ 摘  要

NOVELTY - Electrochemical aptamer sensor comprises polydiallyl dimethyl ammonium chloride, graphene, glassy carbon electrode, nano gold particle and gene fragment. The molecular weight of the polydiallyl dimethyl ammonium chloride is 100,000-200,000. The graphene is a product of graphene oxide reduced by hydrazine hydrate but is not limited to reduced graphene oxide. USE - Electrochemical aptamer sensor for detecting OH-PCB106. ADVANTAGE - The electrochemical aptamer sensor is successfully applied to the detection of hydroxypolychlorinated biphenyls in water samples. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for preparing the electrochemical aptamer sensor, which involves: (1) immobilizing polydiallyldimethylammonium chloride modified graphene on the surface of the glassy carbon electrode; (2) modifying nano gold particles to the electrode surface; (3) immobilizing thiol-modified aptamer that can specifically recognize hydroxypolychlorinated biphenyl 106 (OH-PCB106) on the surface of the modified glassy carbon electrode; (4) preparing electrochemical aptamer sensor for the detection of OH-PCB106.