▎ 摘　　要

NOVELTY - The invention claims a SERS self-reference micro-fluidic chip based on magnetic graphene, comprising substrate, on substrate provided with rear relative feedstock end connected to reaction channel and reference channel, channel response of axis symmetry of substrate and reference channel again and feedstock pipe connected, on first reactant upper sample tube and second sampling tube respectively connected with reactant feedstock tube, SERS substrate material injection channel through micro-valve and micro-flow reaction channel connected; cover sheet and substrate phase matching, magnetic device set on cover plate, magnetic graphene solution flow and magnetic force traction into reaction channel by magnetic device and production. The utility model provides a SERS self-reference micro-fluidic chip based on magnetic graphene, preparation method and application, implement biochemical reaction for SERS real time monitoring of different stage, so as to realize SERS) self-reference) function.