▎ 摘　　要

NOVELTY - Method for detecting sulfonated graphene involves adding 6-carboxyfluorescein-labeled single stranded DNA and target strand DNA to trisaminomethane-hydrochloric acid (Tris-HCL) buffer, performing hybridization reaction, adding sulfonated graphene, reacting at room temperature, and measuring the fluorescence intensity at 520 nm, and excitation wavelength at 494 nm. USE - The method is useful for detecting sulfonated graphene for distinguishing between single base mismatched DNA and target DNA strand (all claimed). DETAILED DESCRIPTION - Method for detecting sulfonated graphene involves adding 6-carboxyfluorescein-labeled single stranded DNA and target strand DNA to trisaminomethane-hydrochloric acid (Tris-HCL) buffer, performing hybridization reaction, adding sulfonated graphene, reacting at room temperature, and measuring the fluorescence intensity at 520 nm, and excitation wavelength at 494 nm. The sulfonated graphene is prepared by dispersing oxidized graphene solid in deionized water, performing ultrasonic reaction for 3 hours to obtain 4 mg/ml oxidized graphene, adding 5 wt.% sodium carbonate solution to the dispersion of graphene oxide to obtain pH at 9-10, then adding 30 ml 0.16 g/ml sodium borohydride solution, uniformly stirring the mixture at 80 degrees C at a speed of 8000 revolutions/minute (rpm) for 1 hour to obtain neutral partial reduced graphene oxide, dispersing the partially reduced partial reduced graphene oxide in deionized water to obtain partially reduced graphene oxide suspension, adding diazonium salt of p-aminobenzenesulfonic acid, uniformly stirring for 2 hours in ice bath, centrifuging, washing precipitate until the pH is 7 to obtain neutral coupling, dispersing the resulting conjugate in 150 ml deionized water, adding 10 ml 1.6 g/ml hydrazine hydrate, uniformly stirring at 100 degrees C for 24 hours for performing reduction reaction, adding 5 wt.% sodium carbonate solution to the precipitate sulfonated graphene in a dropwise manner, washing the prepared sulfonated graphene with double-distilled water, and then vacuum-drying the washed sulfonated graphene solid. INDEPENDENT CLAIMS are included for the following: (1) DNA fluorescence detection kit, comprising liquid (A) comprising sulfonated graphene solution and liquid (B) comprising Tris-HCl buffer and probe FAM-single-stranded DNA; and (2) method for using sulfonated graphene-based DNA fluorescence detection kit involves adding 40 mu l target strand DNA to 445 mu liquid (B), uniformly mixing, reacting the mixture at 37 degrees C for 30 minutes, adding 15 mu l liquid (A), uniformly mixing, reacting at room temperature for 30 seconds, and measuring fluorescence intensity, and excitation wavelength.