▎ 摘　　要

NOVELTY - Preparing a gold-graphene oxide composite nanomaterial, involves preparing a positively charged gold nanoparticle comprising a solution of cetyltrimethylammonium bromide in a concentration of 0.01 mol/L, a chloroformic acid aqueous solution having a concentration of 0.001 mol/L, and an aqueous solution of sodium borohydride having a concentration of 0.1 mol/L are separately prepared. An aqueous solution of hexaalkyltrimethylammonium bromide and an aqueous solution of chloroauric acid are uniformly mixed at room temperature. Adding an aqueous solution of sodium borohydride dropwise to the above mixed solution under stirring, gradually increasing the temperature until boiling, and continuing to stir and stir in a boiling state until the color of the solution turns into wine red, that is, a positively charged gold nanometer particle is prepared. Preparation of negatively charged graphene oxide involves preparing 0.2 mol/L potassium persulfate and 0.4 mol/L phosphorus pentoxide. USE - Gold-graphene oxide composite nanomaterial is used in the quantitative detection of adenosine triphosphate (claimed). ADVANTAGE - The gold-graphene oxide composite nano material can achieve good combination of gold and graphene oxide has good biocompatibility, high stability, and can be used as an effective fluorescent cesium, and elimination of biomolecules for quantitative detection. DETAILED DESCRIPTION - Preparing a gold-graphene oxide composite nanomaterial, involves preparing a positively charged gold nanoparticle comprising a solution of cetyltrimethylammonium bromide in a concentration of 0.01 mol/L, a chloroformic acid aqueous solution having a concentration of 0.001 mol/L, and an aqueous solution of sodium borohydride having a concentration of 0.1 mol/L are separately prepared. An aqueous solution of hexaalkyltrimethylammonium bromide and an aqueous solution of chloroauric acid are uniformly mixed at room temperature. Adding an aqueous solution of sodium borohydride dropwise to the above mixed solution under stirring, gradually increasing the temperature until boiling, and continuing to stir and stir in a boiling state until the color of the solution turns into wine red, that is, a positively charged gold nanometer particle is prepared. Preparation of negatively charged graphene oxide involves preparing a concentration of 0.2 mol/L potassium persulfate and a concentration of 0.4 mol/L phosphorus pentoxide. A certain amount of graphite powder is added to a mixture of potassium persulfate and phosphorus pentoxide under continuous stirring at 80 degrees C, mixed uniformly, filtered, washed, and dried, the mixture is stirred and dispersed to a certain volume at 0 degrees C in sulfuric acid, then slowly added a certain amount of potassium permanganate, reacted at 35 degrees C for 2 hours, added deionized water to dilute, and added a volume of 30% aqueous solution of hydrogen peroxide to obtain graphene oxide. The prepared graphene oxide is washed with a 10 wt.% by mass hydrochloric acid solution and deionized water, and then dried in an oven at 60 degrees C. Preparation of gold-graphene oxide involves preparing an aqueous solution of 1.0 mg/mL graphene oxide, stirring and mixing a certain volume of the aqueous graphene oxide solution and the gold nanoparticle solution at room temperature, then ultrasonically mixing, and then filtering with a filter. The gold-graphene oxide was peeled off from the filter and diluted with distilled water to a desired concentration for storage at a low temperature.